Anti-ARID1B antibody
英文名称 | ARID1B |
中文名称 | 抑癌基因结合蛋白ARID1B抗体 |
别 名 | 6A3 5; ARID 1B; ARID domain containing protein 1B; AT rich interactive domain 1B (SWI1 like); AT rich interactive domain 1B; AT rich interactive domain containing protein 1B; BAF 250b; BAF250b; BRG1 associated factor 250b; BRG1 binding protein; BRG1 binding protein ELD/OSA1; BRG1 binding protein hELD/OSA1; BRIGHT; DAN 15; DAN15; Eld (eyelid)/Osa protein; ELD/OSA1; hELD/OSA1; hOsa 2; hOsa2; KIAA1235; OSA 2; Osa homolog 2; OSA2; OTTHUMP00000040115; p250R; RP11 419L10.1. |
DATASHEET
Host:Rabbit
Target Protein:ARID1B
IR:Immunogen Range:1501-1600/2236
Clonality:Polyclonal
Isotype:IgG
Entrez Gene:57492
Swiss Prot:Q8NFD5
Source:KLH conjugated synthetic peptide derived from human ARID1B:1501-1600/2236
Purification:affinity purified by Protein A
Storage:0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol. Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.
Background:The SWI-SNF complex is involved in the activation of transcription via the remodeling of nucleosome structure in an ATP-dependent manner. Brm (also designated SNF2 alpha) and Brg-1 (also designated SNF2 beta) are the ATPase subunits of the mammalian SWI-SNF complex. Brm, Brg-1, Ini1 (integrase interactor 1, also designated SNF5), BAF155 (also designated SRG3) and BAF170 are thought to comprise the functional core of the SWI-SNF complex. Addition of Ini1, BAF155 and BAF170 to Brg-1 appears to increase remodeling activity. Other complex subunits, such as BAF250 (p270 or ARID 1A) and BAF250b(ARID1B), are thought to play regulatory roles.
Size:100ul
Concentration:1mg/ml
Applications:WB(1:500-2000)
ELISA(1:5000-10000)
IHC-P(1:100-500)
IHC-F(1:100-500)
ICC(1:100-500)
IF(1:100-500)
Cross Reactive Species:Human
Mouse
Rat
Chicken
Dog
Cow
Sheep
.
For research use only. Not intended for diagnostic or therapeutic use.
VALIDATION IMAGES
Tissue/cell: mouse embryo tissue; 4% Paraformaldehyde-fixed and paraffin-embedded;
Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min;
Incubation: Anti-ARID1B Polyclonal Antibody, Unconjugated(bs-12520R) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining
Tissue/cell: Mouse embryos tissue; 4% Paraformaldehyde-fixed and paraffin-embedded;
Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min;
Incubation: Anti-ARID1B Polyclonal Antibody, Unconjugated(bs-12520R) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining
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