Anti-phospho-Cytokeratin 8 (Ser431) antibody (bs-12920R)

Anti-phospho-Cytokeratin 8 (Ser431) antibody

DATASHEET

Host:Rabbit

Target Protein:phospho-Cytokeratin 8 (Ser431)

IR:Immunogen Range:LT(p-S)PG

Clonality:Polyclonal

Isotype:IgG

Entrez Gene:3856

Swiss Prot:P05787

Source:KLH conjugated synthesised phosphopeptide derived from human CK8 around the phosphorylation site of Ser431:LT(p-S)PG 

Purification:affinity purified by Protein A

Storage:0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol. Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.

Background:This gene is a member of the type II keratin family clustered on the long arm of chromosome 12. Type I and type II keratins heteropolymerize to form intermediate-sized filaments in the cytoplasm of epithelial cells. The product of this gene typically dimerizes with keratin 18 to form an intermediate filament in simple single-layered epithelial cells. This protein plays a role in maintaining cellular structural integrity and also functions in signal transduction and cellular differentiation. Mutations in this gene cause cryptogenic cirrhosis. Alternatively spliced transcript variants have been found for this gene. [provided by RefSeq, Jan 2012].

Size:100ul

Concentration:1mg/ml

Applications:WB(1:500-2000)
ELISA(1:5000-10000)
IHC-P(1:100-500)
IHC-F(1:100-500)
IF(1:100-500)

Cross Reactive Species:Human
Mouse
Rat
Horse
.

For research use only. Not intended for diagnostic or therapeutic use.

VALIDATION IMAGES

Sample: Stomach (Mouse) Lysate at 40 ug
Primary: Anti-phospho-Cytokeratin 8 (Ser431) (bs-12920R) at 1/300 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 53 kD
Observed band size: 53 kD

Paraformaldehyde-fixed, paraffin embedded (Human colon cancer); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (p-Cytokeratin 8 (Ser431)) Polyclonal Antibody, Unconjugated (bs-12920R) at 1:500 overnight at 4°C, followed by a conjugated secondary (sp-0023) for 20 minutes and DAB staining.