Anti-phospho-ACTH (Ser168) antibody
| 英文名称 | phospho-ACTH (Ser168) |
| 中文名称 | 磷酸化促肾上腺皮质激素ACTH抗体 |
| 别 名 | ACTH (phospho S168); p-ACTH (phospho S168); ACTH; Adrenocorticotropic hormone; Adrenocorticotropin; Alpha melanocyte stimulating hormone; alpha-MSH; alphaMSH; Beta LPH; Beta melanocyte stimulating hormone; Beta-endorphin; beta-MSH; CLIP; Corticotropin; Corticotropin lipotropin; Corticotropin-like intermediary peptide; Gamma LPH; gamma-MSH; Lipotropin beta; Lipotropin gamma; Lipotropin, included; LPH; Melanocyte-stimulating hormone, included; Melanotropin alpha; Melanotropin beta; Melanotropin gamma; Melanotropin, included; Met-enkephalin; MSH; NPP; POC; POMC; Pomc-1; Pomc1; Pomc2; Pro ACTH endorphin; Pro opiomelanocortin; Pro-opiomelanocortin-alpha; Proopiomelanocortin; Proopiomelanocortin preproprotein; Tetracosactide. |
DATASHEET
Host:Rabbit
Target Protein:phospho-ACTH (Ser168)
IR:Immunogen Range:DE(p-S)AE
Clonality:Polyclonal
Isotype:IgG
Entrez Gene:5443
Swiss Prot:P01189
Source:KLH conjugated synthetic peptide derived from human ACTH:DE(p-S)AE
Purification:affinity purified by Protein A
Storage:0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol. Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.
Background:This gene encodes a polypeptide hormone precursor that undergoes extensive, tissue-specific, post-translational processing via cleavage by subtilisin-like enzymes known as prohormone convertases. There are eight potential cleavage sites within the polypeptide precursor and, depending on tissue type and the available convertases, processing may yield as many as ten biologically active peptides involved in diverse cellular functions. The encoded protein is synthesized mainly in corticotroph cells of the anterior pituitary where four cleavage sites are used; adrenocorticotrophin, essential for normal steroidogenesis and the maintenance of normal adrenal weight, and lipotropin beta are the major end products. In other tissues, including the hypothalamus, placenta, and epithelium, all cleavage sites may be used, giving rise to peptides with roles in pain and energy homeostasis, melanocyte stimulation, and immune modulation. These include several distinct melanotropins, lipotropins, and endorphins that are contained within the adrenocorticotrophin and beta-lipotropin peptides. Mutations in this gene have been associated with early onset obesity, adrenal insufficiency, and red hair pigmentation. Alternatively spliced transcript variants encoding the same protein have been described. [provided by RefSeq, Jul 2008].
Size:100ul
Concentration:1mg/ml
Applications:ELISA(1:5000-10000)
IHC-P(1:100-500)
IHC-F(1:100-500)
ICC(1:100-500)
IF(1:100-500)
Cross Reactive Species:Human
Rat
Dog
.
For research use only. Not intended for diagnostic or therapeutic use.
VALIDATION IMAGES
Paraformaldehyde-fixed, paraffin embedded (Human brain glioma); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (phospho-ACTH (Ser168)) Polyclonal Antibody, Unconjugated (bs-12959R) at 1:400 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
Tissue/cell: rat adrenal tissue; 4% Paraformaldehyde-fixed and paraffin-embedded;
Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min;
Incubation: Anti-phospho-ACTH (Ser168) Polyclonal Antibody, Unconjugated(bs-12959R) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining
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