Anti-ZAR1L antibody (bs-13550R)

Anti-ZAR1L antibody

DATASHEET

Host:Rabbit

Target Protein:ZAR1L

IR:Immunogen Range:51-150/321

Clonality:Polyclonal

Isotype:IgG

Entrez Gene:646799

Swiss Prot:A6NP61

Source:KLH conjugated synthetic peptide derived from human ZAR1L:51-150/321 

Purification:affinity purified by Protein A

Storage:0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol. Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.

Background:This gene encodes a member of the ZAR1 family that is predominantly expressed in oocytes and early embryos. The protein may function as an RNA regulator in early embryos.

Size:100ul

Concentration:1mg/ml

Applications:WB(1:500-2000)
ELISA(1:5000-10000)
IHC-P(1:100-500)
IHC-F(1:100-500)
ICC(1:100-500)
IF(1:100-500)

Cross Reactive Species:Human
.

For research use only. Not intended for diagnostic or therapeutic use.

VALIDATION IMAGES

Sample:A549 (Human)cell Lysate at 40 ug
Primary: Anti-ZAR1L(bs-13550R)at 1/300 dilution
Secondary: IRDye800CW Goat Anti-RabbitIgG at 1/20000 dilution
Predicted band size: 36kD
Observed band size: 36kD

Paraformaldehyde-fixed, paraffin embedded (Human glioma); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (ZAR1L) Polyclonal Antibody, Unconjugated (bs-13550R) at 1:400 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.