Anti-CHIP antibody
| 英文名称 | CHIP |
| 中文名称 | E3泛素蛋白连接酶CHIP蛋白抗体 |
| 别 名 | Antigen NY-CO-7; C terminus of Hsp70-interacting protein; CarboJNC terminus of Hsp70 interacting protein; CarboJNC terminus of Hsp70-interacting protein; CarboJNC terminus of Hsp70p interacting protein; CHIP; CHIP_HUMAN; CLL associated antigen KW 8; CLL-associated antigen KW-8; E3 ubiquitin protein ligase CHIP; Heat shock protein A binding protein 2 (c terminal); HSPABP2; NY CO 7; PP1131; SDCCAG7; Serologically defined colon cancer antigen 7; STIP1 homology and U Box containing protein 1; STIP1 homology and U box containing protein 1 E3 ubiquitin protein ligase; STIP1 homology and U box-containing protein 1; STUB 1; STUB1; UBOX 1; UBOX1. |
DATASHEET
Host:Rabbit
Target Protein:CHIP
IR:Immunogen Range:101-200/303
Clonality:Polyclonal
Isotype:IgG
Entrez Gene:10273
Swiss Prot:Q9UNE7
Source:KLH conjugated synthetic peptide derived from human CHIP:101-200/303
Purification:affinity purified by Protein A
Storage:0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol. Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.
Background:STUB1, or CHIP, is a ubiquitin ligase/cochaperone that participates in protein quality control by targeting a broad range of chaperone protein substrates for degradation.
Size:100ul
Concentration:1mg/ml
Applications:WB(1:500-2000)
ELISA(1:5000-10000)
IHC-P(1:100-500)
IHC-F(1:100-500)
ICC(1:100-500)
IF(1:100-500)
Cross Reactive Species:Human
Mouse
Rat
Chicken
Dog
Pig
Cow
Horse
Sheep
.
For research use only. Not intended for diagnostic or therapeutic use.
VALIDATION IMAGES
Sample:
Hela(Human) Cell Lysate at 30 ug
Primary: Anti-CHIP (bs-15405R) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 35 kD
Observed band size: 35 kD
Paraformaldehyde-fixed, paraffin embedded (rat brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (CHIP) Polyclonal Antibody, Unconjugated (bs-15405R) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
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