Anti-HEG1 antibody (bs-15449R)

Anti-HEG1 antibody 

DATASHEET

Host:Rabbit

Target Protein:HEG1

IR:Immunogen Range:1151-1381/1381

Clonality:Polyclonal

Isotype:IgG

Entrez Gene:57493

Swiss Prot:Q9ULI3

Source:KLH conjugated synthetic peptide derived from human HEG1:1151-1381/1381 

Purification:affinity purified by Protein A

Storage:0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol. Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.

Background:HEG1 (HEG homolog 1) is also known as HEG and is a 1,381 amino acid protein that exists as two alternatively spliced isoforms, one of which is a single-pass membrane protein and the other of which is secreted. HEG1, which is expressed in endothelial cells and smooth muscle cells of heart tissue, contains two EGF-like domains that play a role in calcium-binding events which may regulate concentric heart growth. The gene encoding HEG1 maps to human chromosome 3, which houses over 1,100 genes, including a chemokine receptor (CKR) gene cluster and a variety of human cancer-related gene loci. Key tumor suppressing genes on chromosome 3 include those that encode the apoptosis mediator RASSF1, the cell migration regulator HYAL1 and the angiogenesis suppressor SEMA3B. Marfan Syndrome, porphyria, von Hippel-Lindau syndrome, osteogenesis imperfecta and Charcot-Marie-Tooth Disease are a few of the numerous genetic diseases associated with chromosome 3.

Size:100ul

Concentration:1mg/ml

Applications:ELISA(1:5000-10000)
IHC-P(1:100-500)
IHC-F(1:100-500)
ICC(1:100-500)
IF(1:100-500)

Cross Reactive Species:Human
Mouse
Rat
Dog
Cow
Horse
Rabbit
Sheep
.

For research use only. Not intended for diagnostic or therapeutic use.

VALIDATION IMAGES

Paraformaldehyde-fixed, paraffin embedded (Mouse brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (HEG homolog 1) Polyclonal Antibody, Unconjugated (bs-15449R) at 1:400 overnight at 4°C, followed by a conjugated secondary antibody (sp-0023) for 20 minutes and DAB staining.

Paraformaldehyde-fixed, paraffin embedded (Mouse brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (HEG homolog 1) Polyclonal Antibody, Unconjugated (bs-15449R) at 1:400 overnight at 4°C, followed by a conjugated secondary antibody (bs-0295G-cy3) for 90 minutes, and DAPI for nuclei staining.