Anti-HOXD8 antibody (bs-13600R)

Anti-HOXD8 antibody 

DATASHEET

Host:Rabbit

Target Protein:HOXD8

IR:Immunogen Range:151-250/290

Clonality:Polyclonal

Isotype:IgG

Entrez Gene:3234

Swiss Prot:P13378

Source:KLH conjugated synthetic peptide derived from human HOXD8:151-250/290 

Purification:affinity purified by Protein A

Storage:0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol. Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.

Background:The Hox proteins are a family of transcription factors that play a role in development and cellular differentiation by regulating downstream target genes. Specifically, the Hox proteins direct DNA-protein and protein-protein interactions that assist in determining the morphologic features associated with the anterior-posterior body axis. Hox proteins are involved in controlling axial patterning, leukemias and hereditary malformations. HoxD8 (homeobox D8), also known as HOX4E, is a 290 amino acid protein that localizes to the nucleus and contains one homeobox DNA-binding domain. One of several members of the homeobox superfamily, HoxD8 functions as a sequence-specific transcription factor that is important for the correct positioning of developing limb buds on the anterior-posterior axis.

Size:100ul

Concentration:1mg/ml

Applications:WB(1:500-2000)
ELISA(1:5000-10000)
IHC-P(1:100-500)
IHC-F(1:100-500)
ICC(1:100-500)
IF(1:100-500)

Cross Reactive Species:Human
Mouse
Rat
Pig
Cow
Horse
Sheep
.

For research use only. Not intended for diagnostic or therapeutic use.

VALIDATION IMAGES

Protein: muscle(mouse) lysate at 40ug;
Primary: rabbit Anti-HOXD8 (bs-13600R) at 1:300;
Secondary: HRP conjugated Goat-Anti-rabbit IgG(bs-0295G-HRP) at 1: 5000;
Predicted band size: 32 kD
Observed band size: 34 kD

Tissue/cell: Human colon cancer; 4% Paraformaldehyde-fixed and paraffin-embedded;
Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min;
Incubation: Anti-HOXC8 Polyclonal Antibody, Unconjugated(bs-13600R) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining