Anti-AP1M2 antibody
英文名称 | AP1M2 |
中文名称 | AP-1μ2抗体 |
别 名 | Adaptor protein complex AP-1 mu-2 subunit; Adaptor protein complex AP1 mu2 subunit; Adaptor related protein complex 1 mu2 subunit; Adaptor-related protein complex 1 mu-2 subunit; AP mu chain family member mu1B; AP-1 complex subunit mu-2; AP-mu chain family member mu1B; AP1 complex subunit mu2; AP1M2; AP1M2_HUMAN; AP47 2; Clathrin assembly protein complex 1 medium chain 2; Golgi adaptor HA1/AP1 adaptin mu-2 subunit; Golgi adaptor HA1/AP1 adaptin mu2 subunit; HA1 47 kDa subunit 2; HSMU1B; MU 1B; Mu adaptin 2; Mu-adaptin 2; Mu1B adaptin; Mu1B-adaptin. |
DATASHEET
Host:Rabbit
Target Protein:AP1M2
IR:Immunogen Range:1-100/423
Clonality:Polyclonal
Isotype:IgG
Entrez Gene:10053
Swiss Prot:Q9Y6Q5
Source:KLH conjugated synthetic peptide derived from human AP1M2:1-100/423
Purification:affinity purified by Protein A
Storage:0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol. Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.
Background:Subunit of clathrin-associated adaptor protein complex 1 that plays a role in protein sorting in the trans-Golgi network (TGN) and endosomes. The AP complexes mediate the recruitment of clathrin to membranes and the recognition of sorting signals within the cytosolic tails of transmembrane cargo molecules.
Size:100ul
Concentration:1mg/ml
Applications:WB(1:500-2000)
ELISA(1:5000-10000)
IHC-P(1:100-500)
IHC-F(1:100-500)
ICC(1:100-500)
IF(1:100-500)
Cross Reactive Species:Human
Mouse
Pig
Cow
Horse
Sheep
.
For research use only. Not intended for diagnostic or therapeutic use.
VALIDATION IMAGES
Tissue/cell: human liver carcinoma; 4% Paraformaldehyde-fixed and paraffin-embedded;
Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min;
Incubation: Anti-AP1M2 Polyclonal Antibody, Unconjugated(bs-13638R) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining
Tissue/cell: human liver carcinoma; 4% Paraformaldehyde-fixed and paraffin-embedded;
Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min;
Incubation: Anti-AP1M2 Polyclonal Antibody, Unconjugated(bs-13638R) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining
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