Anti-EpCAM antibody (bs-4889R)

Anti-EpCAM antibody

DATASHEET

Host:Rabbit

Target Protein:EpCAM

IR:Immunogen Range:101-200/314

Clonality:Polyclonal

Isotype:IgG

Entrez Gene:4072

Swiss Prot:P16422

Source:KLH conjugated synthetic peptide derived from human EpCAM:101-200/314 

Purification:affinity purified by Protein A

Storage:0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol. Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.

Background:This gene encodes a carcinoma-associated antigen and is a member of a family that includes at least two type I membrane proteins. This antigen is expressed on most normal epithelial cells and gastrointestinal carcinomas and functions as a homotypic calcium-independent cell adhesion molecule. The antigen is being used as a target for immunotherapy treatment of human carcinomas. Mutations in this gene result in congenital tufting enteropathy. [provided by RefSeq, Dec 2008]

Size:100ul

Concentration:1mg/ml

Applications:WB(1:500-2000)
ELISA(1:5000-10000)
Flow-Cyt(3ug/Test)

Cross Reactive Species:Human
Mouse
Rat
.

For research use only. Not intended for diagnostic or therapeutic use.

VALIDATION IMAGES

Sample:
Lane 1: Large intestine (Mouse) Lysate at 40 ug
Lane 2: Small intestine (Mouse) Lysate at 40 ug
Primary:
Anti-EpCAM (bs-4889R) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 35-45 kD
Observed band size: 38 kD

Blank control: A431.
Primary Antibody (green line): Rabbit Anti-TEpCAM antibody (bs-4889R)
Dilution: 3μg /10^6 cells;
Isotype Control Antibody (orange line): Rabbit IgG .
Secondary Antibody: Goat anti-rabbit IgG-AF647
Dilution: 3μg /test.
Protocol
The cells were incubated in 5%BSA to block non-specific protein-protein interactions for 30 min at at room temperature .Cells stained with Primary Antibody for 30 min at room temperature. The secondary antibody used for 40 min at room temperature. Acquisition of 20,000 events was performed.