Anti-CHRAC1 antibody
| 英文名称 | CHRAC1 |
| 中文名称 | CHRAC1蛋白抗体 |
| 别 名 | CHARC1; CHARC15; CHRAC-1; CHRAC-15; Chrac1; CHRAC15; CHRC1_HUMAN; Chromatin accessibility complex 1; Chromatin accessibility complex 15 kDa protein; Chromatin accessibility complex protein 1; DNA polymerase epsilon subunit p15; histone-fold protein CHRAC15; HuCHRAC15; YCL1. |
DATASHEET
Host:Rabbit
Target Protein:CHRAC1
IR:Immunogen Range:1-100/131
Clonality:Polyclonal
Isotype:IgG
Entrez Gene:54108
Swiss Prot:Q9NRG0
Source:KLH conjugated synthetic peptide derived from human CHRAC1:1-100/131
Purification:affinity purified by Protein A
Storage:0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol. Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.
Background:DNA replication is initiated by the binding of initiation factors to the origin of replication. Nucleosomes inhibit access to the replication machinery at these origin sequences. Nucleosome remodeling factors increase the accessibility of nucleosomal DNA to transcriptional regulators (1). CHRAC15 and CHRAC17 are subunits of the nucleosomal remodeling factor CHRAC (chromatin accessibility complex),which increases the accessibility of nucleosomal DNA in an ATP-dependent manner (2). Unlike other known chromatin remodelling factors, CHRAC also functions during chromatin assembly by using ATP to convert irregular chromatin into a regular array of nucleosomes with even spacing (3). This conversion process occurs when CHRAC organizes randomly deposited histones into a regularly spaced array (4). In the presence of CHRAC, the nucleosomal ATPase ISWI catalyses several ATP-dependent transitions of chromatin structure (5).
Size:100ul
Concentration:1mg/ml
Applications:WB(1:500-2000)
ELISA(1:5000-10000)
IHC-P(1:100-500)
IHC-F(1:100-500)
ICC(1:100-500)
IF(1:100-500)
Cross Reactive Species:Human
Mouse
Rat
Chicken
Cow
Sheep
.
For research use only. Not intended for diagnostic or therapeutic use.
VALIDATION IMAGES
Sample: muscle (Mouse) Lysate at 40 ug
Primary: Anti-CHRAC1(bs-13924R) at 1/300 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 14 kD
Observed band size: 13 kD
Sample:
Testis (Mouse) Lysate at 40 ug
Molt-4(Human) Cell Lysate at 30 ug
U-2OS(Human) Cell Lysate at 30 ug
Primary: Anti- CHRAC1 (bs-13924R) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 14 kD
Observed band size: 14 kD
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