Anti-Melan A antibody
| 英文名称 | Melan A |
| 中文名称 | 黑色素瘤相关抗原/黑色素-A抗体 |
| 别 名 | Protein Melan-A; Antigen LB39 AA; Melanoma HMB45; Antigen SK29 AA; Antigen SK29-AA; CMM 1; CMM; CMM1; Cutaneous Malignant Melanoma Dysplastic Nevus; DNS; Dysplastic Nevus Syndrome; FAMMM; MART1; melan A; Melan A protein; Melanoma antigen recognized by T-cells 1; MLM; Monophenol monooJNCgenase; Tumor rejection antigen AB; tyrosinase; Melanoma HMB45; Melanoma; Melan-A; MART-1; MAR1_HUMAN. |
DATASHEET
Host:Rabbit
Target Protein:Melan A
IR:Immunogen Range:1-80/113
Clonality:Polyclonal
Isotype:IgG
Entrez Gene:77836
Swiss Prot:Q2TA50
Source:KLH conjugated synthetic peptide derived from mouse Melan A:1-80/113
Purification:affinity purified by Protein A
Storage:0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol. Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.
Background:Melanoma-associated antigens recognized by cytotoxic T lymphocytes (CTL) have been grouped into three categories: melanocyte differentiation antigens, cancer/testis-specific antigens and mutated or aberrantly expressed antigens. Many of these antigens consist of peptides that are presented to T cells by HLA molecules; they represent potential targets for cancer immunotherapy. Melan-A (also designated MART-1) is a melanocyte differentiation antigen that is specific to melanomas, melanocyte cell lines and retina. Melan-A peptide is recognized by most HLA-A2-restricted tumor-specific tumor-infiltrating lymphocytes in patients with melanoma. Antimelanoma cytotoxic T lymphocytes can be generated with a Melan-A peptide, implicating Melan-A as a potential candidate for antigen-specific immunotherapy in melanoma patients.
Size:100ul
Concentration:1mg/ml
Applications:WB(1:500-2000)
ELISA(1:5000-10000)
IHC-P(1:100-500)
IHC-F(1:100-500)
ICC(1:100-500)
IF(1:100-500)
Cross Reactive Species:Human
Mouse
Rat
.
For research use only. Not intended for diagnostic or therapeutic use.
VALIDATION IMAGES
Protein: disease lung in mouse lysate;
Primary: rabbit Anti-Melan A (bs-7362R) at 1:300;
Secondary: HRP conjugated Goat-Anti-rabbit IgG(bs-0295G-HRP) at 1: 5000;
Predicted band size: 26 kD
Observed band size: 26 kD
Tissue/cell: human melanoma tissue; 4% Paraformaldehyde-fixed and paraffin-embedded;
Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min;
Incubation: Anti-Melan A Polyclonal Antibody, Unconjugated(bs-7362R) 1:500, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining
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