Anti-COX6B2 antibody (bs-14009R)

Anti-COX6B2 antibody

DATASHEET

Host:Rabbit

Target Protein:COX6B2

IR:Immunogen Range:1-88/88

Clonality:Polyclonal

Isotype:IgG

Entrez Gene:125965

Swiss Prot:Q6YFQ2

Source:KLH conjugated synthetic peptide derived from human COX6B2:1-88/88 

Purification:affinity purified by Protein A

Storage:0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol. Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.

Background:The cytochrome c oxidase (COX) family of proteins function as the final electron donor in the respiratory chain to drive a proton gradient across the inner mitochondrial membrane, ultimately resulting in the production of water. The mammalian COX apoenzyme is a dimer, with each monomer consisting of 13 subunits, some of which are mitochondrial and some of which are nuclear. Localized to the intermembrane space, COX6b2 (Cytochrome c oxidase subunit 6B2), also known as Cytochrome c oxidase subunit VIb isoform 2 and Cancer/testis antigen 59, is a 88 amino acid mitochondrial protein that is responsible for joining the two COX monomers to form the COX dimer. COX6b2 is specifically expressed in testis and is found to be upregulated in certain cancer cell lines.

Size:100ul

Concentration:1mg/ml

Applications:ELISA(1:5000-10000)
IHC-P(1:100-500)
IHC-F(1:100-500)
ICC(1:100-500)
IF(1:100-500)

Cross Reactive Species:Human
Mouse
Rat
Cow
Sheep
.

For research use only. Not intended for diagnostic or therapeutic use.

VALIDATION IMAGES

Tissue/cell: human lung carcinoma; 4% Paraformaldehyde-fixed and paraffin-embedded;
Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min;
Incubation: Anti-COX6B2 Polyclonal Antibody, Unconjugated(bs-14009R) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining

Tissue/cell: Human lung cancer tissue; 4% Paraformaldehyde-fixed and paraffin-embedded;
Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min;
Incubation: Anti-COX6B2 Polyclonal Antibody, Unconjugated(bs-14009R) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining