Anti-Galectin-1 antibody
| 英文名称 | Galectin-1 |
| 中文名称 | 半乳糖凝集素1抗体 |
| 别 名 | 14 kDa laminin-binding protein; 14 kDa lectin; Beta galactoside binding lectin; Beta galactoside binding lectin L 14 I; beta galactoside binding protein; Beta-galactoside-binding lectin L-14-I; Gal 1; Gal-1; Galaptin; Galbp; Galectin; Galectin1; GBP; HBL; HLBP14; HPL; L 14.5; L-14.5; L14; Lactose binding lectin 1; Lactose-binding lectin 1; Lect14; Lectin galactoside binding soluble 1;Lectin galactoside-binding soluble 1; LEG1_HUMAN; LGALS 1; LGALS1; Lgals1 lectin galactose binding soluble 1; MAPK activating protein MP12; Putative MAPK activating protein MP12; Putative MAPK-activating protein PM12; S Lac lectin 1; S-Lac lectin 1. |
DATASHEET
Host:Rabbit
Target Protein:Galectin-1
IR:Immunogen Range:21-100/135
Clonality:Polyclonal
Isotype:IgG
Entrez Gene:3956
Swiss Prot:P09382
Source:KLH conjugated synthetic peptide derived from human Galectin-1:21-100/135
Purification:affinity purified by Protein A
Storage:0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol. Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.
Background:The galectins are a family of beta-galactoside-binding proteins implicated in modulating cell-cell and cell-matrix interactions. This gene product may act as an autocrine negative growth factor that regulates cell proliferation. [provided by RefSeq, Jul 2008].
Size:100ul
Concentration:1mg/ml
Applications:WB(1:500-2000)
ELISA(1:5000-10000)
IHC-P(1:100-500)
IHC-F(1:100-500)
IF(1:100-500)
Cross Reactive Species:Human
Mouse
Rat
Pig
.
For research use only. Not intended for diagnostic or therapeutic use.
VALIDATION IMAGES
Sample:
NIH/3T3(Mouse) Cell Lysate at 30 ug
Primary: Anti-Galectin-1 (bs-10629R) at 1/500 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 15 kD
Observed band size: 15 kD
Antigen r Tissue/cell: human gastric carcinoma; 4% Paraformaldehyde-fixed and paraffin-embedded;
etrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min;
Incubation: Anti-Galectin-1 Polyclonal Antibody, Unconjugated(bs-10629R) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining
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