Anti-Cytokeratin 2e antibody (bs-14184R)

Anti-Cytokeratin 2e antibody

DATASHEET

Host:Rabbit

Target Protein:Cytokeratin 2e

IR:Immunogen Range:251-350/639

Clonality:Polyclonal

Isotype:IgG

Entrez Gene:3849

Swiss Prot:P35908

Source:KLH conjugated synthetic peptide derived from human Cytokeratin 2e:251-350/639 

Purification:affinity purified by Protein A

Storage:0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol. Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.

Background:The protein encoded by this gene is a member of the keratin gene family. The type II cytokeratins consist of basic or neutral proteins which are arranged in pairs of heterotypic keratin chains coexpressed during differentiation of simple and stratified epithelial tissues. This type II cytokeratin is expressed largely in the upper spinous layer of epidermal keratinocytes and mutations in this gene have been associated with bullous congenital ichthyosiform erythroderma. The type II cytokeratins are clustered in a region of chromosome 12q12-q13. [provided by RefSeq, Jul 2008]

Size:100ul

Concentration:1mg/ml

Applications:WB(1:500-2000)
ELISA(1:5000-10000)
IHC-P(1:100-500)
IHC-F(1:100-500)
ICC(1:100-500)
IF(1:100-500)

Cross Reactive Species:Human
.

For research use only. Not intended for diagnostic or therapeutic use.

VALIDATION IMAGES

Sample:
Hela (Human) Cell Lysate at 40 ug
A431 (Human) Cell Lysate at 40 ug
Primary: Anti- Cytokeratin 2e (bs-14184R)at 1/300 dilution
Secondary: IRDye800CW Goat Anti-RabbitIgG at 1/20000 dilution
Predicted band size: 65kD
Observed band size: 70kD

Paraformaldehyde-fixed, paraffin embedded (human cervical); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (Cytokeratin 2e) Polyclonal Antibody, Unconjugated (bs-14184R) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.