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Anti-PI3 Kinase p110 delta antibody (bs-10656R)

Anti-PI3 Kinase p110 delta antibody (bs-10656R)

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Anti-PI3 Kinase p110 delta antibody

英文名称PI3 Kinase p110 delta
中文名称磷脂酰肌醇激酶催化亚单位D抗体
别    名p110D; p110delta; Phosphatidylinositol 3 kinase catalytic delta polypeptide; Phosphatidylinositol 4 5 bisphosphate 3 kinase catalytic subunit delta isoform; Phosphatidylinositol 45 bisphosphate 3 kinase catalytic subunit delta isoform; Phosphoinositide 3 kinase B; Phosphoinositide 3 kinase C; Phosphoinositide 3 kinase catalytic delta polypeptide; PI3 kinase p110 subunit delta; PI3K; PIK3CD; PK3CD; PtdIns 3 kinase p110; PI 3 Kinase p110 delta; PK3CD_HUMAN; Phosphatidylinositol 4,5-bisphosphate 3-kinase catalytic subunit delta isoform; PI3-kinase subunit delta; PI3K-delta; PI3Kdelta; PtdIns-3-kinase subunit delta; Phosphatidylinositol 4,5-bisphosphate 3-kinase 110 kDa catalytic subunit delta; PtdIns-3-kinase subunit p110-delta; p110delta.  

DATASHEET

Host:Rabbit

Target Protein:PI3 Kinase p110 delta

IR:Immunogen Range:51-150/1044

Clonality:Polyclonal

Isotype:IgG

Entrez Gene:5293

Swiss Prot:O00329

Source:KLH conjugated synthetic peptide derived from human PI3 Kinase p110 delta:51-150/1044 

Purification:affinity purified by Protein A

Storage:0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol. Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.

Background:

Size:100ul

Concentration:1mg/ml

Applications:WB(1:500-2000)
ELISA(1:5000-10000)
IHC-P(1:100-500)
IHC-F(1:100-500)
ICC(1:100-500)
IF(1:100-500)

Cross Reactive Species:Human
Mouse
Rat
.

For research use only. Not intended for diagnostic or therapeutic use.

VALIDATION IMAGES

Sample: Thymus (Mouse) Lysate at 40 ug
Primary: Anti-PI3 Kinase p110 delta (bs-10656R) at 1/300 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 115 kD
Observed band size: 115 kD

Tissue/cell: human lung carcinoma; 4% Paraformaldehyde-fixed and paraffin-embedded;
Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min;
Incubation: Anti-PI3 Kinase p110 delta Polyclonal Antibody, Unconjugated(bs-10656R) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining

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