Anti-DEPDC1B/BRCC3 antibody
| 英文名称 | DEPDC1B/BRCC3 |
| 中文名称 | 乳腺癌细胞3抗体 |
| 别 名 | BRCC3; Breast cancer cell 3; DEP domain containing 1B; DEP domain containing protein 1B; FLJ11252; DEP1B_HUMAN; HBV X transactivated gene 8 protein; HBV X-transactivated gene 8 protein; HBV XAg-transactivated protein 8; HBxAg transactivated protein 1; OTTHUMP00000122605; OTTHUMP00000221973; XTP1; XTP8. |
DATASHEET
Host:Rabbit
Target Protein:DEPDC1B/BRCC3
IR:Immunogen Range:451-529/529
Clonality:Polyclonal
Isotype:IgG
Entrez Gene:55789
Swiss Prot:Q8WUY9
Source:KLH conjugated synthetic peptide derived from human DEPDC1B/BRCC3:451-529/529
Purification:affinity purified by Protein A
Storage:0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol. Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.
Background:GTPase activator activity. biological process: intracellular signaling cascade
Size:100ul
Concentration:1mg/ml
Applications:WB(1:500-2000)
ELISA(1:5000-10000)
IHC-P(1:100-500)
IHC-F(1:100-500)
ICC(1:100-500)
IF(1:100-500)
Cross Reactive Species:Human
Mouse
Rat
Cow
Rabbit
.
For research use only. Not intended for diagnostic or therapeutic use.
VALIDATION IMAGES
Sample:
HepG2 Cell (Human) Lysate at 30 ug
A431 Cell (Human) Lysate at 30 ug
Primary: Anti-DEPDC1B/BRCC3 (bs-14278R) at 1/300 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 62kD
Observed band size: 62kD
Tissue/cell: human brain glioma; 4% Paraformaldehyde-fixed and paraffin-embedded;
Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min;
Incubation: Anti-BRCC3 Polyclonal Antibody, Unconjugated(bs-14278R) 1:500, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining
好评度