Anti-DNA Polymerase epsilon p59 antibody
| 英文名称 | DNA Polymerase epsilon p59 |
| 中文名称 | DNA聚合酶εp59/DNA pol εp59抗体 |
| 别 名 | DNA polymerase epsilon subunit 2; DNA polymerase epsilon subunit B; DNA polymerase II subunit 2; DPE 2; DPE2; DPOE2_HUMAN; POLE 2; POLE2; Polymerase (DNA directed) epsilon 2 (p59 subunit); Polymerase (DNA directed) epsilon 2; polymerase (DNA directed), epsilon 2, accessory subunit; Polymerase, DNA, epsilon-2. |
DATASHEET
Host:Rabbit
Target Protein:DNA Polymerase epsilon p59
IR:Immunogen Range:101-200/527
Clonality:Polyclonal
Isotype:IgG
Entrez Gene:5427
Swiss Prot:P56282
Source:KLH conjugated synthetic peptide derived from human DNA Polymerase epsilon p59:101-200/527
Purification:affinity purified by Protein A
Storage:0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol. Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.
Background:DNA replication, recombination and repair, all of which are necessary for genome stability, require the presence of exonucleases
Size:100ul
Concentration:1mg/ml
Applications:ELISA(1:5000-10000)
IHC-P(1:100-500)
IHC-F(1:100-500)
ICC(1:100-500)
IF(1:100-500)
Cross Reactive Species:Human
Mouse
Rat
Cow
Horse
Sheep
.
For research use only. Not intended for diagnostic or therapeutic use.
VALIDATION IMAGES
Tissue/cell: human laryngo carcinoma; 4% Paraformaldehyde-fixed and paraffin-embedded;
Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min;
Incubation: Anti-POLE2 Polyclonal Antibody, Unconjugated(bs-14356R) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining
Tissue/cell: Mouse embryos tissue; 4% Paraformaldehyde-fixed and paraffin-embedded;
Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min;
Incubation: Anti-DNA Polymerase epsilon p59 Polyclonal Antibody, Unconjugated(bs-14356R) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining
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