Anti-DNA Primase antibody
| 英文名称 | DNA Primase |
| 中文名称 | DNA引物酶抗体 |
| 别 名 | dJ422B11.1.1; DNA primase 58kDa subunit; DNA primase large subunit; DNA primase p58; DNA primase subunit p58; p58; Polypeptide 2A p58; PRIM2; PRIM2A; PRI2_HUMAN; Primase DNA polypeptide 2 (58kDa); Primase polpeptide 2A (58kD); Primase polpeptide 2A 58kDa. |
DATASHEET
Host:Rabbit
Target Protein:DNA Primase
IR:Immunogen Range:51-150/509
Clonality:Polyclonal
Isotype:IgG
Entrez Gene:5558
Swiss Prot:P49643
Source:KLH conjugated synthetic peptide derived from human DNA Primase:51-150/509
Purification:affinity purified by Protein A
Storage:0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol. Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.
Background:This gene encodes the 58 kilodalton subunit of DNA primase, an enzyme that plays a key role in the replication of DNA. The encoded protein forms a heterodimer with a 49 kilodalton subunit. This heterodimer functions as a DNA-directed RNA polymerase to synthesize small RNA primers that are used to create Okazaki fragments on the lagging strand of the DNA. [provided by RefSeq, Sep 2013]
Size:100ul
Concentration:1mg/ml
Applications:WB(1:500-2000)
ELISA(1:5000-10000)
IHC-P(1:100-500)
IHC-F(1:100-500)
ICC(1:100-500)
IF(1:100-500)
Cross Reactive Species:Human
Mouse
Rat
Pig
.
For research use only. Not intended for diagnostic or therapeutic use.
VALIDATION IMAGES
Protein: K562(human) lysates at 30ug;
Primary: rabbit Anti-DNA primase (bs-14357R) at 1:300;
Secondary: HRP conjugated Goat-Anti-rabbit IgG(bs-0295G-HRP) at 1: 5000;
Predicted band size:60 kD
Observed band size:60 kD
Sample:
bone (mouse) Lysate at 40 ug
testis (Mouse) Lysate at 40 ug
Primary: Anti-DNA primase (bs-14357R) at 1/300 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 59 kD
Observed band size: 63 kD
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