Anti-phospho-Dnmt3a (Ser390 + Ser393) antibody
| 英文名称 | phospho-Dnmt3a (Ser390 + Ser393) |
| 中文名称 | 磷酸化DNA甲基转移酶-3α抗体 |
| 别 名 | Dnmt3a (phospho S390 + S393); Dnmt3a (phospho Ser390 + Ser393); p-Dnmt3a (phospho S390 + S393); DNA (cytosine 5) methyltransferase 3 alpha; DNA (cytosine 5) methyltransferase 3A; DNA (cytosine-5)-methyltransferase 3A; DNA cytosine methyltransferase 3A2; DNA methyltransferase 3a; DNA methyltransferase HsaIIIA; DNA MTase HsaIIIA; DNM3A_HUMAN; DNMT 3a; DNMT; Dnmt3a; DNMT3A2; M.HsaIIIA; MCMT; OTTHUMP00000201149. |
DATASHEET
Host:Rabbit
Target Protein:phospho-Dnmt3a (Ser390 + Ser393)
IR:Immunogen Range:HD(p-S)DE(p-S)DT
Clonality:Polyclonal
Isotype:IgG
Entrez Gene:1788
Swiss Prot:Q9Y6K1
Source:KLH conjugated Synthesised phosphopeptide derived from human Dnmt3a around the phosphorylation site of Ser390 + Ser393:HD(p-S)DE(p-S)DT
Purification:affinity purified by Protein A
Storage:0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol. Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.
Background:CpG methylation is an epigenetic modification that is important for embryonic development, imprinting, and X-chromosome inactivation. Studies in mice have demonstrated that DNA methylation is required for mammalian development. This gene encodes a DNA methyltransferase that is thought to function in de novo methylation, rather than maintenance methylation. The protein localizes to the cytoplasm and nucleus and its expression is developmentally regulated. Alternative splicing results in multiple transcript variants encoding different isoforms. [provided by RefSeq, Jul 2008]
Size:100ul
Concentration:1mg/ml
Applications:WB(1:500-2000)
ELISA(1:5000-10000)
IHC-P(1:100-500)
Cross Reactive Species:Human
.
For research use only. Not intended for diagnostic or therapeutic use.
VALIDATION IMAGES
Sample:
Lane 1: 293T (Human) Cell Lysate at 30 ug
Lane 2: SH-SY5Y (Human) Cell Lysate at 30 ug
Lane 3: Molt-4 (Human) Cell Lysate at 30 ug
Lane 4: HepG2 (Human) Cell Lysate at 30 ug
Primary: Anti-phospho-Dnmt3a (Ser390 + Ser393) (bs-14399R) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 120 kD
Observed band size: 120 kD
Paraformaldehyde-fixed, paraffin embedded (human gastric); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (phospho-Dnmt3a (Ser390 + Ser393)) Polyclonal Antibody, Unconjugated (bs-14399R) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
好评度