Anti-GRF2 antibody (bs-16311R)

Anti-GRF2 antibody

DATASHEET

Host:Rabbit

Target Protein:GRF2

IR:Immunogen Range:20-120/1237

Clonality:Polyclonal

Isotype:IgG

Entrez Gene:2889

Swiss Prot:O14827

Source:KLH conjugated synthetic peptide derived from human GRF2:20-120/1237 

Purification:affinity purified by Protein A

Storage:0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol. Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.

Background:This gene encodes a human guanine nucleotide exchange factor. It transduces signals from CRK by binding the SH3 domain of CRK, and activating several members of the Ras family of GTPases. This signaling cascade that may be involved in apoptosis, integrin-mediated signal transduction, and cell transformation. Several alternatively spliced transcript variants of this gene have been described, but the full-length nature of some variants has not been determined. [provided by RefSeq, Jul 2008]

Size:100ul

Concentration:1mg/ml

Applications:WB(1:500-2000)
ELISA(1:5000-10000)
IHC-P(1:100-500)
IHC-F(1:100-500)
ICC(1:100-500)
IF(1:100-500)

Cross Reactive Species:Human
Mouse
Rat
Cow
Sheep
.

For research use only. Not intended for diagnostic or therapeutic use.

VALIDATION IMAGES

Sample: Placenta (Mouse) Lysate at 40 ug
Primary: Anti-GRF2 (bs-16311R) at 1/300 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 140 kD
Observed band size: 120 kD

Protein: placenta(mouse) lysate at 40ug;
Primary: rabbit Anti-GRF2 (bs-16311R) at 1:300;
Secondary: HRP conjugated Goat-Anti-rabbit IgG(bs-0295G-HRP) at 1: 5000;
Predicted band size: 140 kD
Observed band size: 140 kD