Anti-TRAF5 antibody (bs-16563R)

Anti-TRAF5 antibody

DATASHEET

Host:Rabbit

Target Protein:TRAF5

IR:Immunogen Range:101-200/557

Clonality:Polyclonal

Isotype:IgG

Entrez Gene:7188

Swiss Prot:O00463

Source:KLH conjugated synthetic peptide derived from human TRAF5:101-200/557 

Purification:affinity purified by Protein A

Storage:0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol. Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.

Background:The scaffold protein encoded by this gene is a member of the tumor necrosis factor receptor-associated factor (TRAF) protein family and contains a meprin and TRAF homology (MATH) domain, a RING-type zinc finger, and two TRAF-type zinc fingers. TRAF proteins are associated with, and mediate signal transduction from members of the TNF receptor superfamily. This protein is one of the components of a multiple protein complex which binds to tumor necrosis factor (TNF) receptor cytoplasmic domains and mediates TNF-induced activation. Alternate transcriptional splice variants have been characterized. [provided by RefSeq, Jul 2008]

Size:100ul

Concentration:1mg/ml

Applications:WB(1:500-2000)
ELISA(1:5000-10000)
IHC-P(1:100-500)
IHC-F(1:100-500)
ICC(1:100-500)
IF(1:100-500)

Cross Reactive Species:Human
Rat
Pig
Horse
.

For research use only. Not intended for diagnostic or therapeutic use.

VALIDATION IMAGES

Sample:
Hela Cell (Human) Lysate at 30 ug
Primary: Anti- TRAF5 (bs-16563R) at 1/300 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 64 kD
Observed band size: 64 kD

Paraformaldehyde-fixed, paraffin embedded (rat prostate); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (TRAF5) Polyclonal Antibody, Unconjugated (bs-16563R) at 1:5000 overnight at 4°C, followed by a conjugated secondary (sp-0023) for 20 minutes and DAB staining.