Anti-phospho-Integrin beta 1 (Tyr795) antibody
| 英文名称 | phospho-Integrin beta 1 (Tyr795) |
| 中文名称 | 磷酸化整合素β1/Integrin β1抗体 |
| 别 名 | Integrin beta 1 (phospho Y795); p-Integrin beta 1 (phospho Tyr795); beta 1 subunit; CD 29; CD29; CD29 antigen; Fibrinogen Receptor beta subunit; Fibronectin receptor subunit beta; FNRB; Glycoprotein IIa; GP IIa; GPIIA; integrin beta 1 (fibronectin receptor beta polypeptide antigen CD29 includes MDF2 MSK12); Integrin beta-1; Integrin VLA 4 subunit beta; integrin VLA-4 beta subunit; Integrin, beta 1 (fibronectin receptor, beta polypeptide, antigen CD29 includes MDF2, MSK12); ITB1_HUMAN; ITGB 1; ITGB1; MDF2; MSK12; OTTHUMP00000019420; Very Late Activation Protein beta Polypeptide; Very late activation protein, beta polypeptide; VLA BETA; VLA-4 subunit beta; VLA-BETA; VLAB; VLAbeta. |
DATASHEET
Host:Rabbit
Target Protein:phospho-Integrin beta 1 (Tyr795)
IR:Immunogen Range:PK(p-Y)EG
Clonality:Polyclonal
Isotype:IgG
Entrez Gene:3688
Swiss Prot:P05556
Source:KLH conjugated synthesised phosphopeptide derived from human Integrin beta 1 around the phosphorylation site of Tyr795:PK(p-Y)EG
Purification:affinity purified by Protein A
Storage:0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol. Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.
Background:Integrins are heterodimeric proteins made up of alpha and beta subunits. At least 18 alpha and 8 beta subunits have been described in mammals. Integrin family members are membrane receptors involved in cell adhesion and recognition in a variety of processes including embryogenesis, hemostasis, tissue repair, immune response and metastatic diffusion of tumor cells. This gene encodes a beta subunit. Multiple alternatively spliced transcript variants which encode different protein isoforms have been found for this gene. [provided by RefSeq, Jul 2008]
Size:100ul
Concentration:1mg/ml
Applications:ELISA(1:5000-10000)
IHC-P(1:100-500)
IHC-F(1:100-500)
Flow-Cyt(1μg/Test)
ICC(1:100)
IF(1:100-500)
Cross Reactive Species:Human
Rat
Dog
Pig
Cow
Rabbit
Sheep
.
For research use only. Not intended for diagnostic or therapeutic use.
VALIDATION IMAGES
Paraformaldehyde-fixed, paraffin embedded (Rat brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (phospho-Integrin beta 1(Tyr795)) Polyclonal Antibody, Unconjugated (bs-16636R) at 1:400 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
Blank control(blue): U251 (fixed with Ice-cold 70% ethanol overnight at 4 °C).
Primary Antibody:Rabbit Anti-phospho-Integrin beta 1(Tyr795) antibody (bs-16636R,Green); Dilution: 1μg in 100 μL 1X PBS containing 0.5% BSA;
Isotype Control Antibody: Rabbit IgG(orange), used under the same conditions;
Secondary Antibody: Goat anti-rabbit IgG-FITC(white blue), Dilution: 1:200 in 1 X PBS containing 0.5% BSA.
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