Anti-KLHL15 antibody (bs-16764R)

Anti-KLHL15 antibody

DATASHEET

Host:Rabbit

Target Protein:KLHL15

IR:Immunogen Range:401-500/604

Clonality:Polyclonal

Isotype:IgG

Entrez Gene:80311

Swiss Prot:Q96M94

Source:KLH conjugated synthetic peptide derived from human KLHL15:401-500/604 

Purification:affinity purified by Protein A

Storage:0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol. Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.

Background:KLHL15 is believed to be a substrate-specific adapter of an E3 ubiquitin-protein ligase complex which regulates the ubiquitination, and subsequent proteasomal degradation, of target proteins. KLHL15 contains one BACK (BTB/Kelch associated) domain, five kelch repeats and one BTB domain. The BTB (broad complex, tramtrack and bric-a-brac) domain, also known as the POZ (poxvirus and zinc finger) domain, is an N-terminal homodimerization domain that contains multiple copies of kelch repeats and/or C2H2-type zinc fingers. Proteins that contain BTB domains are thought to be involved in transcriptional regulation via control of chromatin structure and function.

Size:100ul

Concentration:1mg/ml

Applications:WB(1:500-2000)
ELISA(1:5000-10000)
IHC-P(1:100-500)
IHC-F(1:100-500)
ICC(1:100-500)
IF(1:100-500)

Cross Reactive Species:Human
Mouse
Rat
Pig
Cow
Horse
Rabbit
Sheep
.

For research use only. Not intended for diagnostic or therapeutic use.

VALIDATION IMAGES

Sample: Eye (mouse) Lysate at 40 ug
Primary: Anti-KLHL15(bs-16764R) at 1/300 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 70 kD
Observed band size: 70 kD

Sample: MCF-7 (human)cell Lysate at 40 ug
Primary: Anti-KLHL15(bs-16764R) at 1/300 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 70 kD
Observed band size: 75 kD