Anti-SCARA5 antibody
| 英文名称 | SCARA5 |
| 中文名称 | 清道夫受体A亚型5抗体 |
| 别 名 | FLJ23907; MGC45780; NET33; SCAR5_HUMAN; SCARA 5; scara5; Scavenger receptor class A member 5; Scavenger receptor class A, member 5 (putative); Scavenger receptor class A, member 5; Scavenger receptor hlg; Tesr; Testis expressed scavenger receptor. |
DATASHEET
Host:Rabbit
Target Protein:SCARA5
IR:Immunogen Range:231-330/495
Clonality:Polyclonal
Isotype:IgG
Entrez Gene:286133
Swiss Prot:Q6ZMJ2
Source:KLH conjugated synthetic peptide derived from human SCARA5:231-330/495
Purification:affinity purified by Protein A
Storage:0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol. Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.
Background:SCARA5 is a 495 amino acid cell membrane protein. SCARA5 is expressed at high levels in testis, with highest levels specifically found in specific populations of epithelial cells and Sertoli cells. SCARA5 forms a homotrimer single-pass type II membrane complex present on the surface of the plasma membrane. Cells transfected with SCARA5 were able to bind particular gram-positive and gram-negative bacteria, suggesting a possible role for SCARA5 in immunity to infection. It has also been suggested that SCARA5 may be involved in transferrin delivery pathways. Expressed as four isoforms produced by alternative splicing, SCARA5 contains one collagen-like domain and one SRCR domain.
Size:100ul
Concentration:1mg/ml
Applications:WB(1:500-2000)
ELISA(1:5000-10000)
IHC-P(1:100-500)
IHC-F(1:100-500)
ICC(1:100-500)
IF(1:100-500)
Cross Reactive Species:Human
Mouse
Rat
Cow
Horse
.
For research use only. Not intended for diagnostic or therapeutic use.
VALIDATION IMAGES
Sample:
Heart (Mouse) Lysate at 40 ug
Primary: Anti-SCARA5 (bs-17271R) at 1/300 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 54 kD
Observed band size: 54 kD
Tissue/cell: Mouse liver tissue; 4% Paraformaldehyde-fixed and paraffin-embedded;
Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min;
Incubation: Anti-SCARA5 Polyclonal Antibody, Unconjugated(bs-17271R) 1:500, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining
好评度