Anti-SCIN antibody (bs-17286R)

Anti-SCIN antibody

DATASHEET

Host:Rabbit

Target Protein:SCIN

IR:Immunogen Range:625-715/715

Clonality:Polyclonal

Isotype:IgG

Entrez Gene:85477

Swiss Prot:Q9Y6U3

Source:KLH conjugated synthetic peptide derived from human SCIN:625-715/715 

Purification:affinity purified by Protein A

Storage:0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol. Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.

Background:SCIN (Scinderin) is a Ca(2+) dependent actin filament severing protein that is presumed to have a regulatory function in exocytosis by affecting the organization of the microfilament network underneath the plasma membrane. In vitro SCIN also has barbed end capping and nucleating activities in the presence of Ca(2+).

Size:100ul

Concentration:1mg/ml

Applications:WB(1:500-2000)
ELISA(1:5000-10000)
IHC-P(1:100-500)
IHC-F(1:100-500)
ICC(1:100-500)
IF(1:100-500)

Cross Reactive Species:Human
Mouse
Rat
Dog
Cow
Horse
Sheep
.

For research use only. Not intended for diagnostic or therapeutic use.

VALIDATION IMAGES

Sample:
Kidney (Mouse) Lysate at 40 ug
Primary: Anti- SCIN (bs-17286R) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 80 kD
Observed band size: 86 kD

Paraformaldehyde-fixed, paraffin embedded (rat pancreas); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (SCIN) Polyclonal Antibody, Unconjugated (bs-17286R) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.