Anti-CYT 19 antibody (bs-10633R)

Anti-CYT 19 antibody

DATASHEET

Host:Rabbit

Target Protein:CYT 19

IR:Immunogen Range:21-120/375

Clonality:Polyclonal

Isotype:IgG

Entrez Gene:57412

Swiss Prot:Q9HBK9

Source:KLH conjugated synthetic peptide derived from human CYT 19:21-120/375 

Purification:affinity purified by Protein A

Storage:0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol. Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.

Background:Formation of methylated metabolites is a critical step in the metabolism of inorganic arsenic. Arsenite methyltransferase (cyt19) is localized to the cytoplasm and operates in the transfer of a methyl group from AdoMet to trivalent arsenicals producing methylated and dimethylated arsenicals. It methylates arsenite to form methylarsonate which is reduced to methylarsonite. Methylarsonite acts as a substrate and is converted into a much less toxic compound dimethylarsinate. cyt19 is highly expressed in liver. Inherited variation in cyt19 may contribute to variation in arsenic metabolism and possibly arsenic-dependent carcinogenesis in humans.

Size:100ul

Concentration:1mg/ml

Applications:WB(1:500-2000)
ELISA(1:5000-10000)

Cross Reactive Species:Human
Mouse
Rat
Chicken
Dog
Cow
Horse
Rabbit
Sheep
.

For research use only. Not intended for diagnostic or therapeutic use.

VALIDATION IMAGES

Protein: heart(mouse) lysate at 40ug;
Primary: rabbit Anti-CYT 19 (bs-10633R) at 1:300;
Secondary: HRP conjugated Goat-Anti-rabbit IgG(bs-0295G-HRP) at 1: 5000;
Predicted band size: 42 kD
Observed band size: 42 kD

Sample:
heart (Mouse) Lysate at 40 ug
liver (Mouse) Lysate at 40 ug
Primary: Anti-CYT 19 (bs-10633R) at 1/300 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 42 kD
Observed band size: 48 kD