Anti-OTUD6B antibody (bs-17567R)

Anti-OTUD6B antibody

DATASHEET

Host:Rabbit

Target Protein:OTUD6B

IR:Immunogen Range:51-150/293

Clonality:Polyclonal

Isotype:IgG

Entrez Gene:51633

Swiss Prot:Q8N6M0

Source:KLH conjugated synthetic peptide derived from human OTUD6B:51-150/293 

Purification:affinity purified by Protein A

Storage:0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol. Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.

Background:This gene encodes a member of the ovarian tumor domain (OTU)-containing subfamily of deubiquitinating enzymes. Deubiquitinating enzymes are primarily involved in removing ubiquitin from proteins targeted for degradation. This protein may function as a negative regulator of the cell cycle in B cells. [provided by RefSeq, Nov 2013]

Size:100ul

Concentration:1mg/ml

Applications:WB(1:500-2000)
ELISA(1:5000-10000)
IHC-P(1:100-500)
IHC-F(1:100-500)
ICC(1:100-500)
IF(1:100-500)

Cross Reactive Species:Human
Mouse
Dog
Rabbit
Saccharomyces cerevisiae.

For research use only. Not intended for diagnostic or therapeutic use.

VALIDATION IMAGES

Sample:
Jurkat(Mouse) Cell Lysate at 40 ug
Primary: Anti-OTUD6B (bs-17567R) at 1/300 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 34 kD
Observed band size: 34 kD

Paraformaldehyde-fixed, paraffin embedded (Mouse brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (OTUD6B) Polyclonal Antibody, Unconjugated (bs-17567R) at 1:400 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.