Anti-MOXD1 antibody (bs-17733R)

Anti-MOXD1 antibody

DATASHEET

Host:Rabbit

Target Protein:MOXD1

IR:Immunogen Range:501-600/613

Clonality:Polyclonal

Isotype:IgG

Entrez Gene:26002

Swiss Prot:Q6UVY6

Source:KLH conjugated synthetic peptide derived from human MOXD1:501-600/613 

Purification:affinity purified by Protein A

Storage:0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol. Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.

Background:MOXD1 is a 613 amino acid single-pass type I membrane protein of the Endoplasmic reticulum that belongs to the copper type II ascorbate-dependent monooJNCgenase family. Existing as two alternatively spliced isoforms, MOXD1 is expressed in adult spinal cord, adrenal gland, brain, testis, uterus, lung and kidney, as well as fetal liver and brain. MOXD1 is upregulated during replicative senescence in primary fibroblast and umbilical vein endothelial cell cultures, and uses two copper ions per subunit as a cofactor. MOXD1 contains one DOMON domain, undergoes post-translational N-glycosylation and is encoded by a gene that maps to human chromosome 6. Chromosome 6 contains 170 million base pairs, comprises nearly 6% of the human genome and is associated with early onset intestinal cancer, Porphyria cutanea tarda, Parkinson's disease and Stickler syndrome.

Size:100ul

Concentration:1mg/ml

Applications:WB(1:500-2000)
ELISA(1:5000-10000)
IHC-P(1:100-500)
IHC-F(1:100-500)
ICC(1:100-500)
IF(1:100-500)

Cross Reactive Species:Human
Mouse
Rat
Dog
Pig
Cow
Horse
Rabbit
Sheep
.

For research use only. Not intended for diagnostic or therapeutic use.

VALIDATION IMAGES

Sample:
Jurkat(Human) Cell Lysate at 30 ug
Primary: Anti-MOXD1 (bs-17733R) at 1/300 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 68 kD
Observed band size: 68 kD

Paraformaldehyde-fixed, paraffin embedded (Mouse brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (MOXD1) Polyclonal Antibody, Unconjugated (bs-17733R) at 1:400 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.