Anti-MTHFSD antibody (bs-17880R)

Anti-MTHFSD antibody

DATASHEET

Host:Rabbit

Target Protein:MTHFSD

IR:Immunogen Range:81-180/383

Clonality:Polyclonal

Isotype:IgG

Entrez Gene:64779

Swiss Prot:Q2M296

Source:KLH conjugated synthetic peptide derived from human MTHFS:81-180/383 

Purification:affinity purified by Protein A

Storage:0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol. Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.

Background:MTHFSD (Methenyltetrahydrofolate synthetase domain-containing protein) is a 383 amino acid protein that contains one RRM (RNA recognition motif) domain, which suggests that MTHFSD functions as a RNA-binding protein. The RRM domain consists of four strands and two helices arranged in an α/β sandwich. The gene encoding MTHFSD maps to human chromosome 16, which encodes over 900 genes and comprises nearly 3% of the human genome.

Size:100ul

Concentration:1mg/ml

Applications:WB(1:500-2000)
ELISA(1:5000-10000)
IHC-P(1:100-500)
IHC-F(1:100-500)
ICC(1:100-500)
IF(1:100-500)

Cross Reactive Species:Human
Mouse
Rat
Dog
Cow
Horse
Sheep
.

For research use only. Not intended for diagnostic or therapeutic use.

VALIDATION IMAGES

Sample:
Jurkat(Human) Cell Lysate at 30 ug
Primary: Anti-MTHFSD (bs-17880R) at 1/300 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 42 kD
Observed band size: 42 kD

Paraformaldehyde-fixed, paraffin embedded (mouse brain tissue); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (MTHFSD) Polyclonal Antibody, Unconjugated (bs-17880R) at 1:400 overnight at 4°C, followed by a conjugated secondary (sp-0023) for 20 minutes and DAB staining.