Anti-ornithine aminotransferase antibody (bs-18036R)

Anti-ornithine aminotransferase antibody

DATASHEET

Host:Rabbit

Target Protein:ornithine aminotransferase

IR:Immunogen Range:351-439/439

Clonality:Polyclonal

Isotype:IgG

Entrez Gene:4942

Swiss Prot:P04181

Source:KLH conjugated synthetic peptide derived from human ornithine aminotransferase:351-439/439 

Purification:affinity purified by Protein A

Storage:0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol. Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.

Background:OAT (ornithine aminotransferase (mitochondrial), ornithine-oxo-acid aminotransferase) is a 439 amino acid protein encoded by the human gene OAT. OAT belongs to the class III pyridoxal-phosphate-dependent aminotransferase family and is usually found as a homotetramer in the mitochondrion matrix. OAT catalyzes the major catalytic reaction for ornithine. Ornithinemia, presumably due to deficiency of ornithine ketoacid aminotransferase (OAT) has been found in patients with gyrate atrophy of the choroid and retina. The clinical history of gyrate atrophy is usually night blindness that begins in late childhood, accompanied by sharply demarcated circular areas of chorioretinal atrophy. During the second and third decades the areas of atrophy enlarge. The hepatic cleavage product, hepatic OAT, is formed by cleaving a 25 amino acid transit peptide from the N-terminus of the OAT precursor. The renal form is produced by cleaving a 35 amino acid transit peptide from the N-terminus。

Size:100ul

Concentration:1mg/ml

Applications:WB(1:500-2000)
ELISA(1:5000-10000)
IHC-P(1:100-500)
IHC-F(1:100-500)
ICC(1:100-500)
IF(1:100-500)

Cross Reactive Species:Human
Mouse
Rat
Chicken
Pig
Cow
Horse
.

For research use only. Not intended for diagnostic or therapeutic use.

VALIDATION IMAGES

Sample:
A431(Human) Cell Lysate at 30 ug
Small intestine (Mouse) Lysate at 40 ug
Primary: Anti- ornithine aminotransferase (bs-18036R) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 45 kD
Observed band size: 45 kD

Paraformaldehyde-fixed, paraffin embedded (rat brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (ornithine aminotransferase) Polyclonal Antibody, Unconjugated (bs-18036R) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.