Anti-LSMD1 antibody (bs-18438R)

Anti-LSMD1 antibody

DATASHEET

Host:Rabbit

Target Protein:LSMD1

IR:Immunogen Range:31-125/125

Clonality:Polyclonal

Isotype:IgG

Entrez Gene:84316

Swiss Prot:Q9BRA0

Source:KLH conjugated synthetic peptide derived from human LSMD1:31-125/125 

Purification:affinity purified by Protein A

Storage:0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol. Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.

Background:LSmD1 is a 125 amino acid protein that is a component of the N-terminal acetyltransferase C (NatC) complex. Composed of MAK10, NAT-12 and LSmD1, the NatC complex catalyzes the acetylation of amino-terminal methionine residues. siRNA knockdown of NatC complex subunits leads to p53-dependent cell death and reduced growth of cell lines. There are two isoforms of LSmD1 that are produced as a result of alternative splicing events.

Size:100ul

Concentration:1mg/ml

Applications:ELISA(1:5000-10000)
IHC-P(1:100-500)
IHC-F(1:100-500)
ICC(1:100-500)
IF(1:100-500)

Cross Reactive Species:Human
Mouse
Rat
Pig
Cow
Horse
Rabbit
Sheep
.

For research use only. Not intended for diagnostic or therapeutic use.

VALIDATION IMAGES

Paraformaldehyde-fixed, paraffin embedded (Mouse brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (LSMD1) Polyclonal Antibody, Unconjugated (bs-18438R) at 1:400 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.

Paraformaldehyde-fixed, paraffin embedded (Rat liver); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (LSMD1) Polyclonal Antibody, Unconjugated (bs-18438R) at 1:400 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.