Anti-phospho-N WASP (Tyr256) antibody
| 英文名称 | phospho-N WASP (Tyr256) |
| 中文名称 | 磷酸化神经Wiskott Aldrich综合征蛋白抗体 |
| 别 名 | DKFZp779G0847; MGC48327; N-WASP; Neural Wiskott Aldrich syndrome protein; Neural Wiskott-Aldrich syndrome protein; NWASP; WASL; WASL_HUMAN; Wiskott Aldrich syndrome gene like; Wiskott Aldrich syndrome gene like protein; Wiskott Aldrich syndrome like; WiskottAldrich syndrome like. |
DATASHEET
Host:Rabbit
Target Protein:phospho-N WASP (Tyr256)
IR:Immunogen Range:VI(p-Y)DF
Clonality:Polyclonal
Isotype:IgG
Entrez Gene:8976
Swiss Prot:O00401
Source:KLH conjugated synthesised phosphopeptide derived from human N WASP around the phosphorylation site of Tyr256:VI(p-Y)DF
Purification:affinity purified by Protein A
Storage:0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol. Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.
Background:This gene encodes a member of the Wiskott-Aldrich syndrome (WAS) protein family. Wiskott-Aldrich syndrome proteins share similar domain structure, and associate with a variety of signaling molecules to alter the actin cytoskeleton. The encoded protein is highly expressed in neural tissues, and interacts with several proteins involved in cytoskeletal organization, including cell division control protein 42 (CDC42) and the actin-related protein-2/3 (ARP2/3) complex. The encoded protein may be involved in the formation of long actin microspikes, and in neurite extension. [provided by RefSeq, Jul 2013]
Size:100ul
Concentration:1mg/ml
Applications:ELISA(1:5000-10000)
IHC-P(1:100-500)
IHC-F(1:100-500)
ICC(1:100-500)
IF(1:100-500)
Cross Reactive Species:Human
Mouse
Rat
Dog
Pig
Cow
Horse
Sheep
.
For research use only. Not intended for diagnostic or therapeutic use.
VALIDATION IMAGES
Paraformaldehyde-fixed, paraffin embedded (Mouse brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (phospho-N WASP (Tyr256)) Polyclonal Antibody, Unconjugated (bs-18982R) at 1:400 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
Paraformaldehyde-fixed, paraffin embedded (Rat brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (phospho-N WASP (Tyr256)) Polyclonal Antibody, Unconjugated (bs-18982R) at 1:400 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
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