Anti-Sarcalumenin antibody (bs-19394R)

Anti-Sarcalumenin antibody

DATASHEET

Host:Rabbit

Target Protein:Sarcalumenin

IR:Immunogen Range:451-550/932

Clonality:Polyclonal

Isotype:IgG

Entrez Gene:6345

Swiss Prot:Q86TD4

Source:KLH conjugated synthetic peptide derived from human Sarcalumenin,Isoform 2 :451-550/932 

Purification:affinity purified by Protein A

Storage:0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol. Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.

Background:

Size:100ul

Concentration:1mg/ml

Applications:WB(1:500-2000)
ELISA(1:5000-10000)
IHC-P(1:100-500)
IHC-F(1:100-500)
ICC(1:100-500)
IF(1:100-500)

Cross Reactive Species:Human
Mouse
.

For research use only. Not intended for diagnostic or therapeutic use.

VALIDATION IMAGES

Sample:
Heart (Mouse) Lysate at 40 ug
Muscle (Mouse) Lysate at 40 ug
Skin (Mouse) Lysate at 40 ug
Primary: Anti- Sarcalumenin (bs-19394R) at 1/300 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 97kD
Observed band size: 75 kD

Tissue/cell: human esophageal carcinoma; 4% Paraformaldehyde-fixed and paraffin-embedded;
Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min;
Incubation: Anti-Sarcalumenin Polyclonal Antibody, Unconjugated(bs-19394R) 1:500, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining