Anti-POLR3C antibody
| 英文名称 | POLR3C |
| 中文名称 | DNA指导的RNA聚合酶亚基C抗体 |
| 别 名 | POLR3C; DNA directed III 62 kDa polypeptide; DNA-directed RNA polymerase III subunit C; DNA-directed RNA polymerase III subunit rpc3; Polymerase (RNA) III (DNA directed) polypeptide C (62kD); RNA polymerase C subunit 3; RNA polymerase III 62 kDa subunit; RNA polymerase III C62 subunit; RNA polymerase III subunit C3; RPC3; RPC3_HUMAN. |
DATASHEET
Host:Rabbit
Target Protein:POLR3C
IR:Immunogen Range:21-120/534
Clonality:Polyclonal
Isotype:IgG
Entrez Gene:10623
Swiss Prot:Q9BUI4
Source:KLH conjugated synthetic peptide derived from human POLR3C:21-120/534
Purification:affinity purified by Protein A
Storage:0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol. Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.
Background:
Size:100ul
Concentration:1mg/ml
Applications:WB(1:500-2000)
ELISA(1:5000-10000)
IHC-P(1:100-500)
IHC-F(1:100-500)
ICC(1:100-500)
IF(1:100-500)
Cross Reactive Species:Human
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For research use only. Not intended for diagnostic or therapeutic use.
VALIDATION IMAGES
Protein:
Raji(human) lysate at 40ug;
U937(human) lysate at 40ug;
Primary: rabbit Anti-POLR3C(bs-9569R) at 1:300;
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 61 kD
Observed band size: 61 kD
Tissue/cell: human prostate tissue; 4% Paraformaldehyde-fixed and paraffin-embedded;
Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min;
Incubation: Anti-POLR3C Polyclonal Antibody, Unconjugated(bs-9569R) 1:500, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining
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