Anti-phospho-NFAT2 (Ser233) antibody
| 英文名称 | phospho-NFAT2 (Ser233) |
| 中文名称 | 磷酸化活化T细胞核因子1蛋白抗体 |
| 别 名 | NFAT2 (phospho S233); p-NFAT2 (phospho S233); p-NFAT2 (Ser235 mouse) ; NFAT2; MGC138448; NF ATc; NF ATc1; NFAT 2; NFATc1; NFAT transcription complex cytosolic component; NFATC 1; NFATc; NFATc1; Nuclear factor of activated T cells cytoplasmic 1; Nuclear factor of activated T cells cytoplasmic calcineurin dependent 1; Nuclear factor of activated T cells cytosolic component 1; NFAC1_HUMAN. |
DATASHEET
Host:Rabbit
Target Protein:phospho-NFAT2 (Ser233)
IR:Immunogen Range:LG(p-S)SP
Clonality:Polyclonal
Isotype:IgG
Entrez Gene:4772
Swiss Prot:O95644
Source:KLH conjugated synthesised phosphopeptide derived from human NFAT2 around the phosphorylation site of Ser233:LG(p-S)SP
Purification:affinity purified by Protein A
Storage:0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol. Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.
Background:The protein encoded by this gene is a member of the RecQ DNA helicase family. DNA helicases are enzymes involved in various types of DNA repair, including mismatch repair, nucleotide excision repair and direct repair. Some members of this family are associated with genetic disorders with predisposition to malignancy and chromosomal instability. The biological function of this helicase has not yet been determined. Two alternatively spliced transcripts, which encode the same isoform but differ in their 5' and 3' UTRs, have been described. [provided by RefSeq, Jul 2008]
Size:100ul
Concentration:1mg/ml
Applications:WB(1:500-2000)
ELISA(1:5000-10000)
IHC-P(1:100-500)
IHC-F(1:100-500)
ICC(1:100-500)
IF(1:100-500)
Cross Reactive Species:Human
Mouse
Rat
Pig
Cow
Sheep
.
For research use only. Not intended for diagnostic or therapeutic use.
VALIDATION IMAGES
Sample:
K562(Human) Cell Lysate at 30 ug
Primary: Anti-phospho-NFAT2 (Ser233) (Ser235 mouse)(bs-19785R) at 1/300 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 101 kD
Observed band size: 101 kD
Paraformaldehyde-fixed, paraffin embedded (Rat spleen); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (phospho-NFAT2 (Ser233) (Ser235 mouse)) Polyclonal Antibody, Unconjugated (bs-19785R) at 1:400 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
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