Anti-ZNF385B antibody
英文名称 | ZNF385B |
中文名称 | 锌指蛋白385B抗体 |
别 名 | FLJ25270; Z385B_HUMAN; ZFN533; Zinc finger protein 385B; Zinc finger protein 533; Znf385b; ZNF533. |
DATASHEET
Host:Rabbit
Target Protein:ZNF385B
IR:Immunogen Range:1-100/471
Clonality:Polyclonal
Isotype:IgG
Entrez Gene:151126
Swiss Prot:Q569K4
Source:KLH conjugated synthetic peptide derived from human ZNF385B:1-100/471
Purification:affinity purified by Protein A
Storage:0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol. Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.
Background:
Size:100ul
Concentration:1mg/ml
Applications:WB(1:500-2000)
ELISA(1:5000-10000)
IHC-P(1:100-500)
IHC-F(1:100-500)
Flow-Cyt(2ug/Test)
ICC(1:100-500)
IF(1:100-500)
Cross Reactive Species:Human
Mouse
Rat
Dog
Pig
Cow
Sheep
.
For research use only. Not intended for diagnostic or therapeutic use.
VALIDATION IMAGES
Sample:
Spleen (Mouse) Lysate at 40 ug
Cerebrum (Mouse) Lysate at 40 ug
Primary: Anti-ZNF385B (bs-12809R) at 1/300 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 50 kD
Observed band size: 50 kD
Blank control:293T.
Primary Antibody (green line): Rabbit Anti-ZNF385B antibody (bs-12809R)
Dilution: 2μg /10^6 cells;
Isotype Control Antibody (orange line): Rabbit IgG .
Secondary Antibody : Goat anti-rabbit IgG-AF488
Dilution: 1μg /test.
Protocol
The cells were fixed with 4% PFA (10min at room temperature)and then permeabilized with 90% ice-cold methanol for 20 min at room temperature. The cells were then incubated in 5%BSA to block non-specific protein-protein interactions for 30 min at room temperature .Cells stained with Primary Antibody for 30 min at room temperature. The secondary antibody used for 40 min at room temperature. Acquisition of 20,000 events was performed.
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