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Anti-STCH/HSPA13 antibody (bs-12823R)

Anti-STCH/HSPA13 antibody (bs-12823R)

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Anti-STCH/HSPA13 antibody

英文名称STCH/HSPA13
中文名称热休克蛋白70家族13抗体
别    名Heat shock 70 kDa protein 13; HEAT SHOCK 70-KD PROTEIN 13; heat shock protein 70kDa family, member 13; heat shock protein70kDa family, member13; HSP13_HUMAN; HSPA13; MGC133835; Microsomal stress 70 protein ATPase core; Microsomal stress-70 protein ATPase core; STCH; Stress 70 protein chaperone microsome associated 60 kDa protein; Stress-70 protein chaperone microsome-associated 60 kDa protein.  

DATASHEET

Host:Rabbit

Target Protein:STCH/HSPA13

IR:Immunogen Range:41-140/471

Clonality:Polyclonal

Isotype:IgG

Entrez Gene:6782

Swiss Prot:P48723

Source:KLH conjugated synthetic peptide derived from human STCH/HSPA13:41-140/471 

Purification:affinity purified by Protein A

Storage:0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol. Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.

Background:The protein encoded by this gene is a member of the heat shock protein 70 family and is found associated with microsomes. Members of this protein family play a role in the processing of cytosolic and secretory proteins, as well as in the removal of denatured or incorrectly-folded proteins. The encoded protein contains an ATPase domain and has been shown to associate with a ubiquitin-like protein. [provided by RefSeq, Jul 2008]

Size:100ul

Concentration:1mg/ml

Applications:WB(1:500-2000)
IHC-P(1:100-500)
IHC-F(1:100-500)
ICC(1:100-500)
IF(1:100-500)

Cross Reactive Species:Human
Mouse
Rat
Dog
Pig
Cow
Horse
Rabbit
Sheep
Chimpanzee
.

For research use only. Not intended for diagnostic or therapeutic use.

VALIDATION IMAGES

Sample: Hela Cell Lysate at 40 ug
Primary: Anti-STCH/HSPA13 (bs-12823R) at 1/300 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 50 kD
Observed band size: 50 kD

Paraformaldehyde-fixed, paraffin embedded (Mouse brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (STCH) Polyclonal Antibody, Unconjugated (bs-12823R) at 1:400 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.

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