Anti-KDM6B antibody (bs-20004R)

Anti-KDM6B antibody 

DATASHEET

Host:Rabbit

Target Protein:KDM6B

IR:Immunogen Range:1471-1570/1643

Clonality:Polyclonal

Isotype:IgG

Entrez Gene:23135

Swiss Prot:O15054

Source:KLH conjugated synthetic peptide derived from human KDM6B:1471-1570/1643 

Purification:affinity purified by Protein A

Storage:Preservative: 15mM Sodium Azide, Constituents: 1% BSA, 0.01M PBS, pH 7.4 Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.

Background:JMJD3 is a 1,679 amino acid nuclear protein that contains one JmjC domain and belongs to the highly conserved JmjC domain-containing protein family. Functioning as a histone demethylase, JMJD3 uses iron and ascorbate as cofactors to demethylate dimethylated and trimethylated Lys-27 residues of Histone H3, thereby playing an important role in the modification of the histone code. Additionally, JMJD3 regulates posterior development and is involved in the inflammatory response, specifically by mediating macrophage differentiation. JMJD3 is also thought to control the expression of neurogenesis-related proteins and, via this regulatory mechanism, may be necessary for neural commitment during early development. Two isoforms of JMJD3 exist due to alternative splicing events.

Size:100ul

Concentration:1mg/ml

Applications:WB(1:500-2000)
ELISA(1:5000-10000)
IHC-P(1:100-500)
IHC-F(1:100-500)
ICC(1:100-500)
IF(1:100-500)

Cross Reactive Species:Human
Mouse
Rat
Pig
Cow
Horse
Rabbit
Sheep
.

For research use only. Not intended for diagnostic or therapeutic use.

VALIDATION IMAGES

Sample: Cerebellum(Rat) Tissue Lysate at 40 ug
Primary: Anti-KDM6B(bs-20004R)at 1:300 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1:20000 dilution
Predicted band size: 177 kD
Observed band size: 177 kD

Sample:
Cerebellum (Rat) Lysate at 40 ug
Primary: Anti-KDM6B (bs-20004R) at 1/300 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 177 kD
Observed band size: 177 kD