Anti-OLFML1 antibody (bs-21082R)

Anti-OLFML1 antibody

DATASHEET

Host:Rabbit

Target Protein:OLFML1

IR:Immunogen Range:321-402/402

Clonality:Polyclonal

Isotype:IgG

Entrez Gene:283298

Swiss Prot:Q6UWY5

Source:KLH conjugated synthetic peptide derived from human OLFML1:321-402/402 

Purification:affinity purified by Protein A

Storage:Preservative: 15mM Sodium Azide, Constituents: 1% BSA, 0.01M PBS, pH 7.4 Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.

Background:OLFML1 (Olfactomedin-like protein 1) contains 1 olfactomedin-like domain. The specific function of this protein remains unknown.

Size:100ul

Concentration:1mg/ml

Applications:WB(1:500-2000)
ELISA(1:5000-10000)
IHC-P(1:100-500)
IHC-F(1:100-500)
ICC(1:100-500)
IF(1:100-500)

Cross Reactive Species:Human
Mouse
Rat
Dog
Horse
Rabbit
Sheep
.

For research use only. Not intended for diagnostic or therapeutic use.

VALIDATION IMAGES

Sample:
Liver (Mouse) Lysate at 40 ug
Lung (Mouse) Lysate at 40 ug
Eye (Mouse) Lysate at 40 ug
Hippocampus (Mouse) Lysate at 40 ug
Primary: Anti- OLFML1 (bs-21082R) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 46 kD
Observed band size: 48 kD

Paraformaldehyde-fixed, paraffin embedded (mouse brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (OLFML1) Polyclonal Antibody, Unconjugated (bs-21082R) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.