Anti-Smac antibody
| 英文名称 | Smac |
| 中文名称 | 线粒体促凋亡蛋白抗体 |
| 别 名 | Smac / Diablo; 0610041G12Rik; DBLOH_HUMAN; DBOH; DBOH; diablo; Diablo homolog (Drosophila); Diablo homolog (Drosophila); Diablo homolog; Diablo homolog Drosophila; Diablo homolog mitochondrial; Diablo homolog mitochondrial precursor; DIABLO S; DIABLO S; DIABLOS; Direct IAP binding protein with low pI; Direct IAP-binding protein with low pI; FLJ10537; FLJ25049; mitochondrial; Mitochondrial Smac protein; Second Mitochondria Derived Activator of Caspase; Second mitochondria-derived activator of caspase; SMAC 3; smac; Smac protein; SMAC3. |
DATASHEET
Host:Rabbit
Target Protein:Smac
IR:Immunogen Range:151-239/239
Clonality:Polyclonal
Isotype:IgG
Entrez Gene:56616
Swiss Prot:Q9NR28
Source:KLH conjugated synthetic peptide derived from human Smac:151-239/239
Purification:affinity purified by Protein A
Storage:Preservative: 15mM Sodium Azide, Constituents: 1% BSA, 0.01M PBS, pH 7.4 Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.
Background:This gene encodes an inhibitor of apoptosis protein (IAP)-binding protein. The encoded mitochondrial protein enters the cytosol when cells undergo apoptosis, and it moderates the caspase inhibition of IAPs. Multiple polyadenylation sites have been found for this gene. Several alternatively spliced transcript variants that encode distinct isoforms have been described for this gene but the validity of some transcripts, and their predicted ORFs, has not been determined conclusively. The inhibitor of apoptosis (IAP) proteins regulate programmed cell death by inhibiting members of the caspase family of enzymes. A novel mammalian protein that binds to IAPs and neutralizes their inhibitory effect on caspases has been designated Smac/DIABLO. This is a mitochondrial protein that is released along with cytochrome c during apoptosis and activates the cytochrome c/Apaf-1/caspase-9 pathway. Analysis of the structural basis of Smac/DIABLO reveals that the N-terminal amino acids are required for binding of Smac/DIABLO to IAPs and activation of caspases. Smac/DIABLO is expressed in a variety of human and mouse tissues.
Size:100ul
Concentration:1mg/ml
Applications:WB(1:500-2000)
ICC(1:100)
Cross Reactive Species:Human
Mouse
Rat
.
For research use only. Not intended for diagnostic or therapeutic use.
VALIDATION IMAGES
Sample:
Lane 1: Kidney (Mouse) Lysate at 40 ug
Lane 2: Testis (Mouse) Lysate at 40 ug
Primary: Anti-alpha smooth muscle Actin (bs-10196R) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 21 kD
Observed band size: 19 kD
Tissue/cell:SH-SY5Y cell; 4% Paraformaldehyde-fixed; Triton X-100 at room temperature for 20 min; Blocking buffer (normal goat serum,C-0005) at 37°C for 20 min; Antibody incubation with (Smac) polyclonal Antibody, Unconjugated (bs-0667R) 1:100, 90 minutes at 37°C; followed by a FITC conjugated Goat Anti-Rabbit IgG antibody at 37°C for 90 minutes, DAPI (blue, C02-04002) was used to stain the cell nuclei.
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