Anti-IL-10 antibody (bs-20373R)

Anti-IL-10 antibody

DATASHEET

Host:Rabbit

Target Protein:IL-10

IR:Immunogen Range:51-100/178

Clonality:Polyclonal

Isotype:IgG

Entrez Gene:3586

Swiss Prot:P22301

Source:KLH conjugated synthetic peptide derived from human IL-10:51-100/178 

Purification:affinity purified by Protein A

Storage:Preservative: 15mM Sodium Azide, Constituents: 1% BSA, 0.01M PBS, pH 7.4 Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.

Background:IL-10 protein is a cytokine produced primarily by monocytes and to a lesser extent by lymphocytes. This cytokine has pleiotropic effects in immunoregulation and inflammation. It down-regulates the expression of Th1 cytokines, MHC class II Ags, and costimulatory molecules on macrophages. It also enhances B cell survival, proliferation, and antibody production. This cytokine can block NF-kappa B activity, and is involved in the regulation of the JAK-STAT signaling pathway. Knockout studies in mice suggested the function of this cytokine as an essential immunoregulator in the intestinal tract.

Size:100ul

Concentration:1mg/ml

Applications:WB(1:500-2000)

Cross Reactive Species:Human
Mouse
Rat
.

For research use only. Not intended for diagnostic or therapeutic use.

VALIDATION IMAGES

Sample:
Plasma (Rat) at 40 ug
Plasma (Rat) at 40 ug
Primary: Anti-IL-10 (bs-20373R) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 20 kD
Observed band size: 20 kD

Sample:
Raw264.7 (Mouse) Lysate at 30 ug
Primary: Anti-IL-10 (bs-20373R) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 20 kD
Observed band size: 20 kD