Anti-FRA1/FOSL1 antibody (bs-20451R)

Anti-FRA1/FOSL1 antibody

DATASHEET

Host:Rabbit

Target Protein:FRA1/FOSL1

IR:Immunogen Range:101-200/271

Clonality:Polyclonal

Isotype:IgG

Entrez Gene:8061

Swiss Prot:P15407

Source:KLH conjugated synthetic peptide derived from human FRA1/FOSL1:101-200/271 

Purification:affinity purified by Protein A

Storage:Preservative: 15mM Sodium Azide, Constituents: 1% BSA, 0.01M PBS, pH 7.4 Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.

Background:The Fos gene family consists of 4 members: FOS, FOSB, FOSL1, and FOSL2. These genes encode leucine zipper proteins that can dimerize with proteins of the JUN family, thereby forming the transcription factor complex AP-1. As such, the FOS proteins have been implicated as regulators of cell proliferation, differentiation, and transformation. [provided by RefSeq].

Size:100ul

Concentration:1mg/ml

Applications:WB(1:500-2000)
ELISA(1:5000-10000)
IHC-P(1:100-500)
IHC-F(1:100-500)
ICC(1:100-500)
IF(1:100-500)

Cross Reactive Species:Human
Mouse
Rat
Dog
Cow
Horse
Sheep
.

For research use only. Not intended for diagnostic or therapeutic use.

VALIDATION IMAGES

Sample: DU145 Cell (Human) Lysate at 40 ug
Primary: Anti-FRA1/FOSL1 (bs-20451R) at 1/300 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 30 kD
Observed band size: 30 kD

Paraformaldehyde-fixed, paraffin embedded (mouse skin tissue); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (FRA1) Polyclonal Antibody, Unconjugated (bs-20451R) at 1:400 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.