Anti-C9orf3 antibody (bs-20376R)

Anti-C9orf3 antibody

DATASHEET

Host:Rabbit

Target Protein:C9orf3

IR:Immunogen Range:201-300/819

Clonality:Polyclonal

Isotype:IgG

Entrez Gene:84909

Swiss Prot:Q8N6M6

Source:KLH conjugated synthetic peptide derived from human C9orf3:201-300/819 

Purification:affinity purified by Protein A

Storage:Preservative: 15mM Sodium Azide, Constituents: 1% BSA, 0.01M PBS, pH 7.4 Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.

Background:C9orf3 is an aminopeptidase and belongs to the M1 family of metallopeptidases or gluzincins. Aminopeptidases catalyze the hydrolysis of amino acid residues from the N-terminus of peptide or protein substrates. C9orf3 is able to cleave angiotensin III to generate angiotensin IV, a bioactive peptide of the renin-angiotensin pathway but not able to cleave angiotensin I and angiotensin II. It may play a role in the proteolytic processing of bioactive peptides in tissues where it is expressed. C9orf3 is predominantly expressed in pancreas, placenta, liver, testis and heart and at lower level in brain, lung and kidney. There are four named isoforms.

Size:100ul

Concentration:1mg/ml

Applications:WB(1:500-2000)
ELISA(1:5000-10000)
IHC-P(1:100-500)
IHC-F(1:100-500)
ICC(1:100-500)
IF(1:100-500)

Cross Reactive Species:Human
Mouse
Rat
Chicken
.

For research use only. Not intended for diagnostic or therapeutic use.

VALIDATION IMAGES

Sample:Placenta (Mouse)Lysate at 40 ug
Primary: Anti-C9orf3(bs-20376R)at 1/300 dilution
Secondary: IRDye800CW Goat Anti-RabbitIgG at 1/20000 dilution
Predicted band size: 94kD
Observed band size: 94kD

Paraformaldehyde-fixed, paraffin embedded (Rat testis); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (C9orf3) Polyclonal Antibody, Unconjugated (bs-20376R) at 1:400 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructions and DAB staining.