Anti-ZO-1 antibody (bs-23086R)

Anti-ZO-1 antibody

DATASHEET

Host:Rabbit

Target Protein:ZO-1

IR:Immunogen Range:1451-1550/1748

Clonality:Polyclonal

Isotype:IgG

Entrez Gene:7082

Swiss Prot:Q07157

Source:KLH conjugated synthetic peptide derived from human ZO-1:1451-1550/1748 

Purification:affinity purified by Protein A

Storage:Preservative: 15mM Sodium Azide, Constituents: 1% BSA, 0.01M PBS, pH 7.4 Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.

Background:This gene encodes a protein located on a cytoplasmic membrane surface of intercellular tight junctions. The encoded protein may be involved in signal transduction at cell-cell junctions. Two transcript variants encoding distinct isoforms have been identified for this gene. The N-terminal may be involved in transducing a signal required for tight junction assembly, while the C-terminal may have specific properties of tight junctions. The alpha domain might be involved in stabilizing junctions.

Size:100ul

Concentration:1mg/ml

Applications:ELISA(1:5000-10000)
Flow-Cyt(1μg/Test)

Cross Reactive Species:Human
Mouse
Dog
.

For research use only. Not intended for diagnostic or therapeutic use.

VALIDATION IMAGES

Blank control (blue line): U251 (fixed with 70% ethanol (Overnight at 4℃) and then permeabilized with 0.1% Tween for 20 min at room temperature.). Primary Antibody (green line): Rabbit Anti-ZO-1 antibody (bs-23086R),Dilution: 0.2μg /10^6 cells; Isotype Control Antibody (orange line): Rabbit IgG(bs-0295P) . Secondary Antibody (white blue line): Goat anti-rabbit IgG-FITC(bs-0295G-FITC),Dilution: 1μg /test.

Blank control (blue line): U251 (fixed with 70% ethanol (Overnight at 4℃) and then permeabilized with 90% ice-cold methanol for 30 min on ice).
Primary Antibody (green line): Rabbit Anti-ZO-1 antibody (bs-23086R),Dilution: 0.2μg /10^6 cells;
Isotype Control Antibody (orange line): Rabbit IgG .
Secondary Antibody (white blue line): Goat anti-rabbit IgG-FITC,Dilution: 1μg /test.