Anti-CNOT6 antibody (bs-10719R)

Anti-CNOT6 antibody

DATASHEET

Host:Rabbit

Target Protein:CNOT6

IR:Immunogen Range:221-320/557

Clonality:Polyclonal

Isotype:IgG

Entrez Gene:57472

Swiss Prot:Q9ULM6

Source:KLH conjugated synthetic peptide derived from human CNOT6:221-320/557 

Purification:affinity purified by Protein A

Storage:Preservative: 15mM Sodium Azide, Constituents: 1% BSA, 0.01M PBS, pH 7.4 Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.

Background:CNOT6 is a widely expressed subunit of the CCR4-NOT transcription complex. The CCR4-NOT complex is an evolutionarily conserved, multi-component complex known to be involved in transcription as well as mRNA degradation. Various subunits (e.g. CNOT1, CNOT3) are involved in influencing nuclear hormone receptor activities. The CCR4-NOT complex is also involved in the regulation of Histone H3 lysine 4 methylation through a ubiquitin-dependent pathway that likely involves the proteasome. CNOT6 belongs to the CCR4/nocturin family and contains three LRR (leucine-rich) repeats. In the cytoplasm, CNOT6 acts as a poly(A) nuclease involved in mRNA decay mediated by the major-protein-coding determinant of instability (mCRD) of the Fos gene.

Size:100ul

Concentration:1mg/ml

Applications:WB(1:500-2000)
IHC-P(1:100-500)
IHC-F(1:100-500)
ICC(1:100-500)
IF(1:100-500)

Cross Reactive Species:Human
Mouse
Rat
Chicken
Pig
Cow
Horse
Rabbit
Sheep
.

For research use only. Not intended for diagnostic or therapeutic use.

VALIDATION IMAGES

Sample:
Lymph node (Mouse) Lysate at 40 ug
Bone (Mouse) Lysate at 40 ug
Primary: Anti-CNOT6 (bs-10719R) at 1/300 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 63 kD
Observed band size: 63 kD

Paraformaldehyde-fixed, paraffin embedded (Mouse brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (CNOT6) Polyclonal Antibody, Unconjugated (bs-10719R) at 1:400 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructions and DAB staining.