Anti-FoxP3 antibody (bs-23074R)

Anti-FoxP3 antibody

DATASHEET

Host:Rabbit

Target Protein:FoxP3

IR:Immunogen Range:351-431/431

Clonality:Polyclonal

Isotype:IgG

Entrez Gene:50943

Swiss Prot:Q9BZS1

Source:KLH conjugated synthetic peptide derived from human FoxP3:351-431/431 

Purification:affinity purified by Protein A

Storage:0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol. Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.

Background:The protein encoded by this gene is a member of the forkhead/winged-helix family of transcriptional regulators. Defects in this gene are the cause of immunodeficiency polyendocrinopathy, enteropathy, X-linked syndrome (IPEX), also known as X-linked autoimmunity-immunodeficiency syndrome. Alternatively spliced transcript variants encoding different isoforms have been identified. [provided by RefSeq, Jul 2008].

Size:100ul

Concentration:1mg/ml

Applications:WB(1:500-2000)
ELISA(1:5000-10000)
Flow-Cyt(1ug/Test)

Cross Reactive Species:Human
Mouse
Rat
Dog
Pig
Cow
Horse
Sheep
.

For research use only. Not intended for diagnostic or therapeutic use.

VALIDATION IMAGES

Sample:
Lane 1: Thymus (Mouse) Lysate at 40 ug
Lane 2: Spleen (Mouse) Lysate at 40 ug
Lane 3: Lymph node (Mouse) Lysate at 40 ug
Lane 4: Small intestine (Mouse) Lysate at 40 ug
Lane 5: Thymus (Rat) Lysate at 40 ug
Lane 6: Spleen (Rat) Lysate at 40 ug
Lane 7: Lymph node (Rat) Lysate at 40 ug
Lane 8: Small intestine (Rat) Lysate at 40 ug
Primary: Anti-FoxP3 (bs-23074R) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 43/45 kD
Observed band size: 45 kD

Blank control: MCF7.
Primary Antibody (green line): Rabbit Anti-FoxP3 antibody (bs-23074R)
Dilution: 2μg /10^6 cells;
Isotype Control Antibody (orange line): Rabbit IgG .
Secondary Antibody : Goat anti-rabbit IgG-AF647
Dilution: 1μg /test.
Protocol
The cells were fixed with 4% PFA (10min at room temperature)and then permeabilized with 90% ice-cold methanol for 20 min at-20℃.The cells were then incubated in 5%BSA to block non-specific protein-protein interactions for 30 min at room temperature .Cells stained with Primary Antibody for 30 min at room temperature. The secondary antibody used for 40 min at room temperature. Acquisition of 20,000 events was performed.