Anti-phospho-GNA12 (Ser40) antibody
英文名称 | phospho-GNA12 (Ser40) |
中文名称 | 磷酸化鸟苷酸调节蛋白12抗体 |
别 名 | GNA12(phospho-Ser40); GNA12(phospho Ser40); GNA12(phospho S40); P-GNA12(phospho S40); G alpha 12; gep; GNA12; Guanine nucleotide binding protein alpha 12 subunit; NNX3; RMP; AI414047; AI504261; ALPHA SUBUNIT OF THE G12 FAMILY OF HETEROTRIMERIC GS; FLJ21929; G PROTEIN ALPHA 12; MGC104623; MGC130498; MGC99644; GNA12_HUMAN. |
DATASHEET
Host:Rabbit
Target Protein:phospho-GNA12 (Ser40)
IR:Immunogen Range:RR(p-S)RD
Clonality:Polyclonal
Isotype:IgG
Entrez Gene:2768
Swiss Prot:Q03113
Source:KLH conjugated synthetic peptide derived from human GNA12:RR(p-S)RD
Purification:affinity purified by Protein A
Storage:0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol. Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.
Background:
Size:100ul
Concentration:1mg/ml
Applications:WB(1:500-2000)
ELISA(1:5000-10000)
IHC-P(1:100-500)
IHC-F(1:100-500)
ICC(1:100-500)
IF(1:100-500)
Cross Reactive Species:Human
Mouse
Rat
Cow
Rabbit
Sheep
.
For research use only. Not intended for diagnostic or therapeutic use.
VALIDATION IMAGES
Sample:
DU145(Human) Cell Lysate at 30 ug
Primary: Anti-phospho-GNA12(Ser40) (bs-8812R) at 1/300 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 44 kD
Observed band size: 44 kD
Paraformaldehyde-fixed, paraffin embedded (human brain glioma); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (GNA12(Ser40)) Polyclonal Antibody, Unconjugated (bs-8812R) at 1:400 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
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