Anti-MyoD1/Myf3 antibody (bs-23809R)

Anti-MyoD1/Myf3 antibody

DATASHEET

Host:Rabbit

Target Protein:MyoD1/Myf3

IR:Immunogen Range:1-100/320

Clonality:Polyclonal

Isotype:IgG

Entrez Gene:4654

Swiss Prot:P15172

Source:KLH conjugated synthetic peptide derived from human MyoD1/Myf3:1-100/320 

Purification:affinity purified by Protein A

Storage:0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol. Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.

Background:This gene encodes a nuclear protein that belongs to the basic helix-loop-helix family of transcription factors and the myogenic factors subfamily. It regulates muscle cell differentiation by inducing cell cycle arrest, a prerequisite for myogenic initiation. The protein is also involved in muscle regeneration. It activates its own transcription which may stabilize commitment to myogenesis. [provided by RefSeq, Jul 2008]

Size:100ul

Concentration:1mg/ml

Applications:WB(1:500-2000)
ELISA(1:5000-10000)
IHC-P(1:100-500)
IHC-F(1:100-500)
ICC(1:100-500)
IF(1:100-500)

Cross Reactive Species:Human
Mouse
Rat
Dog
Pig
Cow
Horse
Sheep
.

For research use only. Not intended for diagnostic or therapeutic use.

VALIDATION IMAGES

Sample:
Lane 1: Skeletal muscle (Mouse) Lysate at 40 ug
Lane 2: Skeletal muscle (Rat) Lysate at 40 ug
Primary: Anti-MyoD1/Myf3 (bs-23809R) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 40 kD
Observed band size: 40 kD

Sample:
Lane 1: Liver (Mouse) Lysate at 40 ug
Lane 2: Heart (Mouse) Lysate at 40 ug
Primary: Anti-MyoD1’Myf3 (bs-23809R) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 40 kD
Observed band size: 40 kD