Anti-AFP antibody (bs-4822R)

Anti-AFP antibody 

DATASHEET

Host:Rabbit

Target Protein:AFP

IR:Immunogen Range:

Clonality:Polyclonal

Isotype:IgG

Entrez Gene:174

Swiss Prot:P02771

Source:KLH conjugated synthetic peptide derived from human AFP : 

Purification:affinity purified by Protein A

Storage:0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol. Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.

Background:Alpha 1 Fetoprotein is a major plasma protein produced by the yolk sac and the liver during fetal life. Alpha fetoprotein expression in adults is often associated with hepatoma or teratoma. However, hereditary persistance of alpha-fetoprotein may also be found in individuals with no obvious pathology. The protein is thought to be the fetal counterpart of serum albumin, and the alpha fetoprotein and albumin genes are present in tandem in the same transcriptional orientation on chromosome 4. Alpha fetoprotein is found in monomeric as well as dimeric and trimeric forms, and binds copper, nickel, fatty acids and bilirubin. The level of alpha fetoprotein in amniotic fluid is used to measure renal loss of protein to screen for spina bifida and anencephaly. Expression has been documented in human adrenal, liver, ovary, testis, and pancreas. ESTs have been isolated from normal human brain, liver/spleen, embryo and uterus tissue libraries.

Size:100ul

Concentration:1mg/ml

Applications:WB(1:500-2000)

Cross Reactive Species:Human
Mouse
.

For research use only. Not intended for diagnostic or therapeutic use.

VALIDATION IMAGES

Sample:
HepG2 (Human) Cell Lysate at 30 ug
Testis (Mouse) Lysate at 40 ug
Primary: Anti- AFP (bs-4822R) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 65 kD
Observed band size: 65 kD

Sample:
Liver (Mouse) Lysate at 40 ug
Placenta (Mouse) Lysate at 40 ug
Primary: Anti-AFP (bs-4822R) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 65 kD
Observed band size: 70 kD