Anti-RSL1D1 antibody (bs-22952R)

Anti-RSL1D1 antibody

DATASHEET

Host:Rabbit

Target Protein:RSL1D1

IR:Immunogen Range:311-410/452

Clonality:Polyclonal

Isotype:IgG

Entrez Gene:66409

Swiss Prot:Q8BVY0

Source:KLH conjugated synthetic peptide derived from mouse RSL1D1:311-410/452 

Purification:affinity purified by Protein A

Storage:0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol. Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.

Background:RSL1D1, also known as CATX-11, PBK1, L12 or CSIG, is a 490 amino acid nuclear protein that belongs to the ribosomal protein L1P family. Expressed in placenta, RSL1D1 contains many phosphorylated amino acid residues and is encoded by a gene that maps to human chromosome 16p13.13. Chromosome 16 encodes over 900 genes in approximately 90 million base pairs, makes up nearly 3% of human cellular DNA and is associated with a variety of genetic disorders. The GAN gene is located on chromosome 16 and, with mutation, may lead to giant axonal neuropathy, a nervous system disorder characterized by increasing malfunction with growth. The rare disorder Rubinstein-Taybi syndrome is also associated with chromosome 16, though through the CREBBP gene which encodes a critical CREB binding protein. Signs of Rubinstein-Taybi include mental retardation and predisposition to tumor growth and white blood cell neoplasias.

Size:100ul

Concentration:1mg/ml

Applications:WB(1:500-2000)
IHC-P(1:400-800)

Cross Reactive Species:Mouse
Rat
.

For research use only. Not intended for diagnostic or therapeutic use.

VALIDATION IMAGES

Sample:
Lane 1: Heart (Rat) Lysate at 40 ug
Lane 2: Heart (Mouse) Lysate at 40 ug
Lane 3: Muscle (Rat) Lysate at 40 ug
Lane 4: Muscle (Mouse) Lysate at 40 ug
Lane 5: Placenta (Rat) Lysate at 40 ug
Lane 6: Placenta (Mouse) Lysate at 40 ug
Lane 7: Cerebrum (Rat) Lysate at 40 ug
Lane 8: Cerebrum (Mouse) Lysate at 40 ug
Lane 9: Testis (Rat) Lysate at 40 ug
Lane 10: Testis (Mouse) Lysate at 40 ug
Lane 11: Uterus (Rat) Lysate at 40 ug
Lane 12: Uterus (Mouse) Lysate at 40 ug
Primary: Anti-RSL1D1 (bs-22952R) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 55 kD
Observed band size: 52 kD

Paraformaldehyde-fixed, paraffin embedded (rat uterus); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (RSL1D1) Polyclonal Antibody, Unconjugated (bs-22952R ) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.